EPR INVESTIGATIONS OF IRON SIDEROPHORE CHELATES

铁铁载体螯合物的 EPR 研究

• 批准号: 7956665
• 负责人: KEITH A EARLE
• 金额: $ 0.49万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956665
• 关键词: Animals; Binding; Complement; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Growth; Human; Infection; Institution; Investigation; Iron; Ligands; Link; Nature; Property; Proteins; Pseudomonas aeruginosa; Research; Research Personnel; Resources; Serum; Services; Siderophores; Source; Stimulus; Structure; Technology; United States National Institutes of Health; Virulence; cold temperature; pyochelin; pyoverdin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Siderophores are bacterial products which bind iron and increase the rate of bacterial iron transport. Some iron-binding compounds are also known to function in media containing animal or human serum. Since iron sequestration is a known stimulus of bacterial growth during infections, siderophore synthesis has been linked to bacterial virulence. Pseudomonas aeruginosa produces two siderophores under iron-limiting conditions with different chelating properties, pyoverdine (PVD) and pyochelin (PCH). In order to elucidate the nature of the iron siderophore ligands in the two P. Aeruginosa siderophores, we are undertaking low temperature 9.5 GHz EPR studies which are particularly sensitive to the details of the iron ligand structure. This service project complements requests from other center users who wish to use center resources for studies of iron-protein interactions.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 铁载体是结合铁并增加细菌铁转运速率的细菌产物。还已知一些铁结合化合物在含有动物或人血清的培养基中发挥作用。 由于铁螯合是感染过程中细菌生长的一种已知刺激物，因此铁载体合成与细菌毒力有关。 铜绿假单胞菌在铁限制条件下产生两种具有不同螯合特性的铁载体：pyoverdine (PVD) 和pyochelin (PCH)。 为了阐明两种铜绿假单胞菌铁载体中铁铁载体配体的性质，我们正在进行低温 9.5 GHz EPR 研究，该研究对铁配体结构的细节特别敏感。 该服务项目补充了其他希望使用中心资源进行铁-蛋白质相互作用研究的中心用户的请求。