Electron

电子

• 批准号: 7945053
• 负责人: JEFFREY L SALISBURY
• 金额: $ 10.19万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-17 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7945053
• 关键词: Accounting; Antibodies; Archives; Budgets; Cancer Center; Clinic; Color; Comprehensive Cancer Center; Cryopreservation; Cryoultramicrotomy; Custom; Development; Electron Microscopy; Electron Microscopy Shared Resource; Electron Probe Microanalysis; Electrons; Elements; Expenditure; Floor; Gold; Gold Colloid; Grant; Image; Image Analysis; Immunoelectron Microscopy; Individual; Ions; Label; Laboratories; Ligands; Maps; Mayo Clinic Cancer Center; Methodology; Methods; Microscope; Microscopy; Microtomy; Negative Staining; Operative Surgical Procedures; Preparation; Printing; Procedures; Process; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Roentgen Rays; Scanning; Scanning Electron Microscopy; Screening procedure; Services; Shadowing (Histology); Specimen; Specimen Handling; Techniques; Thick; Three-Dimensional Image; Training; Transmission Electron Microscopy; Ultrastructural Pathology; Vacuum; cost; digital; digital imaging; discount; electron tomography; evaporation; image processing; member; microwave electromagnetic radiation; novel; particle; tissue processing; transmission process

The Electron Microscopy Shared Resource (EMSR) is an institutional research resource laboratory providing specimen preparation, microscopy and digital imaging services to investigators within the Mayo Clinic. Centrally located in the Guggenheim Research Building on the 14th floor on the Rochester Campus, the shared resource is fully equipped to offer standard transmission (TEM) and scanning (SEM) electron microscopy. The shared resource also performs X-ray probe microanalysis, electron tomography, cryoultramicrotomy, and immuno-gold labeling procedures. Costs are recovered primarily through recharges to investigator budgets and through institutional support. The facility has been an important shared resource of the Mayo Clinic Cancer Center (MCCC) since 2004. The requested budget will support, in part, current EMSR services utilized by cancer center members, and partially offset the resource's projected yearly operational expenditures of $1,202,039. Usage of the EMSR by MCCC members account for an average utilization of -20% of the EMSR's services and operations during the period from 2003 to 2007. The budget requested for the next five year period will support 8% of the resource's expenses and operations provided to MCCC members. The EMSR provides additional value to Mayo Clinic Cancer Center members in three ways: First the EMSR is continuously developing and implementing new techniques and procedures in electron microscopy directed specifically to the individual needs of MCCC Members. New methods under development include electron tomography, cryo ultramicroscopy, 3-D image analysis, electron diffraction and microwave processing. Second, MCCC member projects are given first priority in the laboratory's specimen handling queue. And finally, MCCC member projects are directly subsidized through discounts.

电子显微镜共享资源(EMSR)是一个机构研究资源实验室，提供 为梅奥诊所的研究人员提供标本制备、显微镜和数字成像服务。 位于罗切斯特校区14楼古根海姆研究大楼的中央位置， 共享资源配备齐全，可提供标准传输(TEM)和扫描(SEM)电子 显微镜。共享资源还执行X射线探针微分析、电子断层扫描、 冷冻显微手术和免疫金标记术。成本主要通过充值来收回 调查人员的预算和机构支助。该设施一直是重要的共享资源 自2004年起在梅奥临床癌症中心(MCCC)工作。 申请的预算将部分支持癌症中心成员目前使用的EMSR服务，以及 部分抵消资源预计年度业务支出1202039美元。EMSR的使用 MCCC成员占EMSR服务和运营的平均利用率为-20% 2003年至2007年期间。请拨的下一个五年期预算将支持8%的 提供给MCCC成员的资源费用和运营。 EMSR通过三种方式为梅奥诊所癌症中心成员提供额外价值：第一，EMSR 正在不断开发和实施电子显微镜领域的新技术和新程序 专门针对MCCC成员的个人需求。正在开发的新方法包括 电子断层扫描、低温超微结构、三维图像分析、电子衍射和微波 正在处理。第二，MCCC成员项目在实验室的标本处理中被优先考虑 排队。最后，MCCC成员项目通过折扣直接获得补贴。