Electron

电子显微镜共享资源（EMSR）是一个机构研究资源实验室，提供 为马约诊所的研究者提供标本制备、显微镜和数字成像服务。 位于罗切斯特校区14楼古根海姆研究大楼的中心， 共享资源配备齐全，可提供标准透射（TEM）和扫描（SEM）电子 显微镜共享资源还执行X射线探针显微分析，电子断层扫描， 冷冻切片术和免疫金标记程序。成本主要通过充值回收 调查员预算和机构支持。该设施一直是重要的共享资源 马约诊所癌症中心（MCCC）的主任。 申请的预算将部分支持癌症中心成员目前使用的EMSR服务， 部分抵消了该资源预计的年度业务支出1 202 039美元。EMSR的使用 MCCC成员的平均使用率为EMSR服务和运营的-20% 在2003年至2007年期间，下一个五年期的预算将支持8%的 提供给MCCC成员的资源的费用和操作。 EMSR通过三种方式为马约诊所癌症中心成员提供额外价值： 不断开发和实施电子显微镜的新技术和新程序 特别针对MCCC会员的个人需求。正在开发的新方法包括 电子层析成像、低温超显微镜、三维图像分析、电子衍射和微波 处理.第二，MCCC成员项目在实验室的标本处理方面享有优先权 队列最后，MCCC成员项目通过折扣直接补贴。

The Electron Microscopy Shared Resource (EMSR) is an institutional research resource laboratory providing specimen preparation, microscopy and digital imaging services to investigators within the Mayo Clinic. Centrally located in the Guggenheim Research Building on the 14th floor on the Rochester Campus, the shared resource is fully equipped to offer standard transmission (TEM) and scanning (SEM) electron microscopy. The shared resource also performs X-ray probe microanalysis, electron tomography, cryoultramicrotomy, and immuno-gold labeling procedures. Costs are recovered primarily through recharges to investigator budgets and through institutional support. The facility has been an important shared resource of the Mayo Clinic Cancer Center (MCCC) since 2004. The requested budget will support, in part, current EMSR services utilized by cancer center members, and partially offset the resource's projected yearly operational expenditures of $1,202,039. Usage of the EMSR by MCCC members account for an average utilization of -20% of the EMSR's services and operations during the period from 2003 to 2007. The budget requested for the next five year period will support 8% of the resource's expenses and operations provided to MCCC members. The EMSR provides additional value to Mayo Clinic Cancer Center members in three ways: First the EMSR is continuously developing and implementing new techniques and procedures in electron microscopy directed specifically to the individual needs of MCCC Members. New methods under development include electron tomography, cryo ultramicroscopy, 3-D image analysis, electron diffraction and microwave processing. Second, MCCC member projects are given first priority in the laboratory's specimen handling queue. And finally, MCCC member projects are directly subsidized through discounts.