CORE--ELECTRON MICROSCOPY

电子显微镜核心设施(EMCF)是一个研究资源实验室，为梅奥诊所基金会内临床和基础科学实验室的研究人员提供标本准备、显微镜、摄影和数字成像服务。该设施配备齐全，可提供标准的透射电子显微镜(TEM)和扫描电子显微镜(SEM)。该设施还进行X射线探头微量分析和免疫金标记程序。与这一核心相关的成本主要通过在适度的机构支持下重新充电来收回。 EMCF请求专门为癌症中心成员开发新的电子显微镜技术和程序提供支持。此外，电子断层扫描、冷冻显微镜和在超微结构水平上识别和定位的新方法正在被纳入EMCF的现有服务。这些方法的结合将使癌症中心的成员能够以以前无法达到的分辨率和保存度研究细胞和分子的三维结构特征。

The Electron Microscopy Core Facility (EMCF) is a research resource laboratory providing specimen preparation, microscopy, photography and digital imaging services to investigators from both clinical and basic science laboratories within the Mayo Clinic Foundation. The facility is fully equipped to offer standard transmission (TEM) and scanning (SEM) electron microscopy. The facility also performs X-ray probe microanalysis and immuno-gold labeling procedures. Costs associated with this core are recovered primarily through recharges with modest institutional support. The EMCF is requesting support specifically for the development of new techniques and procedures in electron microscopy for Cancer Center Members. In addition, methods in electron tomography, cryomicroscopy, and novel methods for identification and localization at the ultrastructural level are being incorporated into the available services of the EMCF. Incorporation of these methodologies will enable Cancer Center members to study three-dimensional structural features of cells and molecules at a resolution and preservation that were previously unattainable.