CORE--ELECTRON MICROSCOPY

核心--电子显微镜

• 批准号: 6989965
• 负责人: JEFFREY L SALISBURY
• 金额: $ 5.91万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989965
• 关键词: biomedical facility; computer data analysis; cryoelectron microscopy; electron microscopy; health science research support; immunoelectron microscopy; neoplasm /cancer; photography; scanning electron microscopy; three dimensional imaging /topography; tissue /cell preparation; tomography; transmission electron microscopy

The Electron Microscopy Core Facility (EMCF) is a research resource laboratory providing specimen preparation, microscopy, photography and digital imaging services to investigators from both clinical and basic science laboratories within the Mayo Clinic Foundation. The facility is fully equipped to offer standard transmission (TEM) and scanning (SEM) electron microscopy. The facility also performs X-ray probe microanalysis and immuno-gold labeling procedures. Costs associated with this core are recovered primarily through recharges with modest institutional support. The EMCF is requesting support specifically for the development of new techniques and procedures in electron microscopy for Cancer Center Members. In addition, methods in electron tomography, cryomicroscopy, and novel methods for identification and localization at the ultrastructural level are being incorporated into the available services of the EMCF. Incorporation of these methodologies will enable Cancer Center members to study three-dimensional structural features of cells and molecules at a resolution and preservation that were previously unattainable.

电子显微镜核心设施（EMCF）是一个研究资源实验室，为来自马约诊所基金会临床和基础科学实验室的研究人员提供标本制备、显微镜检查、摄影和数字成像服务。该设施设备齐全，可提供标准透射（TEM）和扫描（SEM）电子显微镜。该设施还进行X射线探针微量分析和免疫金标记程序。与这一核心资源有关的费用主要通过补充回收，并得到少量机构支助。 EMCF正在请求支持，特别是为癌症中心成员开发电子显微镜的新技术和程序。此外，电子断层扫描、低温显微镜检查和超微结构水平识别和定位的新方法正在纳入EMCF的可用服务。这些方法的结合将使癌症中心的成员能够以以前无法实现的分辨率和保存来研究细胞和分子的三维结构特征。