Shared Resources: Transgenic Facility

共享资源：转基因设施

• 批准号: 7944605
• 负责人: TONY R. HUNTER
• 金额: $ 27.21万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-14 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944605
• 关键词: Animals; Area; Autopsy; Bacteriology; Beds; Biohazardous Substance; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cells; Cryopreservation; DNA; Derivation procedure; Diagnostic; Embryo; Environment; Evaluation; Female; Fertilization; Fertilization in Vitro; Funding; Head; Health; Hour; House mice; Housing; Human Resources; Implant; In Vitro; Inbred Strains Mice; Incubators; Infection; Knock-out; Knockout Mice; Laboratories; Laboratory Animals; Lasers; Masks; Microinjections; Microscope; Monitor; Mus; Needles; Operative Surgical Procedures; Pathologist; Pest Control; Preparation; Procedures; Protective Clothing; Qualifying; Research; Research Personnel; Resistance; Resource Sharing; Rodent; Sentinel; Serologic tests; Services; Shoes; Soil; Specialist; Staging; Surgical Instruments; System; Techniques; Training; Transgenic Organisms; Veterinarians; Water; animal care; animal facility; animal resource; behavior test; blastocyst; embryo cell; embryonic stem cell; male; micromanipulator; mouse model; pathogen; prevent; programs; stem; tissue culture; vibration

Transgenic and knockout mice are an integral part of the research programs of the Cancer Center. The transgenic core laboratory provides a variety of services for investigators, including microinjection of embryonic stem (ES) cells into blastocysts, microinjection of DMA into one-cell embryos, lentiviral infection of two-cell stage embryos, in-vitro fertilization (IVF), cryopreservation and re-derivation of mouse lines, providing neo-resistant mice for generating feeder cells for ES cell culture and providing inbred strains of mice.

转基因和基因敲除小鼠是癌症中心研究计划的一个组成部分。的 转基因核心实验室为研究人员提供各种服务，包括显微注射 胚胎干（ES）细胞进入囊胚，显微注射DMA进入单细胞胚胎，慢病毒感染 二细胞期胚胎、体外受精（IVF）、冷冻保存和小鼠品系的再衍生， 提供用于产生用于ES细胞培养的饲养细胞的新抗性小鼠， 小鼠