ELECTRICALLY HEALING RESPIRATORY EPITHELIAL DAMAGE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 目标1.测定猴呼吸道上皮细胞单层培养和呼吸道上皮器官培养的内源性创伤电场。我们将从淘汰的恒河猴（3只年轻的成年猴，雄性或雌性）中获得气道（气管和支气管组织）。我们将在解剖显微镜下用手术刀制作小切口上皮伤口，并用振动探针测量内源性伤口电流。将上皮上无伤口的组织片段用作对照。我们也将在灵长类呼吸道上皮细胞单层培养物上制造伤口，并使用振动探针系统来测定所产生的内源性伤口电场。 目标2.电刺激促进呼吸道上皮细胞创伤愈合。我们首先将电场应用于灵长类动物呼吸道上皮细胞培养，以确定它们对电场的反应。我们还将监测灵长类动物呼吸道上皮细胞单层的抓伤愈合与延时视频显微镜。我们将如目标1中那样在气管中产生小裂缝上皮伤口，并使用药理学试剂（氨茶碱、SP 600125、双香豆素、Na（3）VO（4））操纵伤口电场。我们将在药物治疗后用振动探针测量内源性伤口电场。我们还将确定这些药物在单层和组织培养中对伤口愈合的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aim 1. To measure endogenous wound electric fields in monolayer cultures of respiratory epithelial cells and in organ cultures of respiratory tract epithelia from monkey. We will obtain airway (tracheal and bronchial tissues) from culled Rhesus monkey (3 young adult monkeys either male or female). We will make small slit epithelial wounds with a scalpel under a dissecting microscope and measure the endogenous wound electric currents with a vibrating probe. Segments of tissues without wound on the epithelium will be used as a control. We will also make wounds on monolayer cultures of primate respiratory epithelial cells and use vibrating probe system to determine endogenous wound electric field generated. Aim 2. To enhance wound healing in respiratory epithelia electrically. We will first apply electric fields to primate respiratory tract epithelial cells in culture to determine their response to electric fields. We will also monitor scratch wound healing of primate respiratory tract epithelial cell monolayers with time-lapse video microscopy. We will generate small slit epithelial wounds in trachea as in Aim 1 and manipulate the wound electric fields with pharmacological agents (Aminophylline, SP 600125, dicumarol, Na(3)VO(4)). We will measure the endogenous wound electric fields with the vibrating probe after drug treatment. We will also determine the effects of these drugs on wound healing in monolayer and tissue cultures.