Examination of Odorant Receptors in Vector Mosquitoes

媒介蚊子气味受体的检查

• 批准号: 7783331
• 负责人: LAURENCE J ZWIEBEL
• 金额: $ 52.25万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7783331
• 关键词: Address; Adult; Aedes; Affect; African; Amino Acids; Anopheles gambiae; Arboviruses; Behavior; Behavioral; Biochemical; Blood; Cell Culture Techniques; Characteristics; Chemicals; Chimera organism; Code; Cues; Culicidae; Data; Dengue; Development; Disease; Elements; Encephalitis; Event; Family; Female; Funding; Generations; Goals; Heart; Homologous Gene; Human; Individual; Insect Vectors; Insecta; Investigation; Larva; Life; Ligands; Light; Link; Location; Malaria; Mediating; Molecular; Mosquito Control; Mutagenesis; Neurons; Odorant Receptors; Odors; Olfactory Receptor Neurons; Oviposition; Patient currently pregnant; Perception; Pheromone; Play; Process; Resources; Role; Scanning; Semiochemicals; Sensory; Series; Signal Pathway; Signal Transduction; Site; Smell Perception; Species Specificity; Specificity; Staging; Stimulus; Structure-Activity Relationship; System; Testing; Variant; Water; West Nile virus; Yellow Fever; aqueous; base; behavior influence; design; disease transmission; disorder control; egg; feeding; human disease; in vivo; insight; novel; novel strategies; odorant-binding protein; pathogen; preference; programs; public health relevance; receptor; research study; response; vector; vector mosquito

DESCRIPTION (provided by applicant): Chemosensory responses are critical components in the control of several essential behaviors of insects that are vectors for pathogens responsible for many important human diseases. In particular, olfaction plays a major role in host seeking and oviposition selection behaviors of blood-feeding female mosquitoes and, as such, constitutes a critical component of the mosquito's ability to transmit diseases such as malaria, dengue, yellow fever and West Nile virus encephalitis. Within this context, and together with our colleagues, we have undertaken a molecular and cellular examination of several elements of the olfactory signal transduction cascade in the principal African malaria vector mosquito Anopheles gambiae sensu stricto and the arbovirus vector Aedes aegypti. An increased understanding of olfactory mechanisms and their underlying chemical cues in these systems may provide insight into the processes of insect behavioral responses in general and disease transmission by vectors in particular and would likely be instrumental in the development of novel mosquito control strategies. We have made significant progress in the characterization of several aspects of olfactory process in both mosquito systems leading to, among other things, the identification and initial characterizations of a family of odorant receptor proteins in An. gambiae (AgORs) and Ae. aegypti (AaORs) that lie at the heart of the olfactory signaling pathway. Building on those advances, this proposal for competitive renewal focuses on extending several elements of our ongoing program including characterization of: (1) structure/function relationships between AgORs and AaORs, (2) the role of AgORs and cognate odorant binding proteins (AgOBPs) in the context of larval olfaction and odor coding in vivo and (3) the molecular events underlying oviposition site selection by gravid adult females. PUBLIC HEALTH RELEVANCE: We have undertaken a molecular and cellular examination of elements of the sense of smell (olfaction) that largely drive several important behaviors in malaria and arbovirus vector mosquitoes. An increased understanding of olfactory mechanisms and their underlying chemical cues in these systems may provide insight into the processes of mosquito behavioral responses and disease transmission by vectors. This information would likely be instrumental in the development of novel mosquito control strategies. Thus far, we have made significant progress and now propose to extend, our characterization of several aspects of olfactory process in both mosquito systems that focus on the role and interactions of a large family of odorant receptor proteins in An. gambiae (AgORs) and Ae. aegypti (AaORs) that lie at the heart of the olfactory signaling pathway.

描述（由申请人提供）：化学感应反应是控制昆虫的几种基本行为的关键组成部分，这些昆虫是导致许多重要人类疾病的病原体的媒介。尤其是，嗅觉在寻求血液喂养雌性蚊子的宿主和产卵选择行为中起着重要作用，因此构成了蚊子传播诸如疟疾，登革热，黄热病和西尼罗病毒病毒的疾病能力的关键组成部分。在这种情况下，与我们的同事一起，我们对主要的非洲疟疾媒介蚊子Anopheles gambiae sensu stricto和arbovirus vector vector aedees aegypti进行了分子和细胞检查。对这些系统中的嗅觉机制及其基本化学提示的越来越多的理解可能会洞悉一般的昆虫行为反应的过程，尤其是向量传播的昆虫行为反应的过程，并且很可能有助于开发新型的蚊子控制策略。在两个蚊子系统中，我们在表征嗅觉过程的几个方面取得了重大进展，从而导致An中的气味受体蛋白家族的鉴定和初始特征。冈比亚（Agors）和AE。位于嗅觉信号通路中心的埃及（AAOR）。在这些进步的基础上，这项竞争性更新的提议着重于扩展我们正在进行的计划的几个要素，包括：（1）：（1）搅拌器与AAORS之间的结构/功能关系，（2）（2）通过在幼虫元素和范围内的范围内，agors和同源性生物结合蛋白（AGOBP）的作用，以及在vivo和femor graver中的上下文中，（3）成人ofors ofer septive the Morecult of the Morecult of of the Morecult of of the Morecult of of the Morecult the Morecult。 公共卫生相关性：我们已经对嗅觉感（嗅觉）元素进行了分子和细胞检查，该元素在很大程度上驱动了疟疾和arbovirus载体媒介蚊子的几种重要行为。对这些系统中嗅觉机制及其基本化学线索的了解越来越多，可能会深入了解蚊子行为反应和媒介传播的疾病传播过程。这些信息可能有助于发展新型蚊子控制策略。到目前为止，我们已经取得了重大进展，现在提议扩展，我们对两个蚊子系统中嗅觉过程的几个方面的表征，侧重于AN中大型杂质受体蛋白的作用和相互作用。冈比亚（Agors）和AE。位于嗅觉信号通路中心的埃及（AAOR）。