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Characterization of Anopheline Odorant Receptors

按蚊气味受体的表征

基本信息

批准号：
9552301
负责人：
LAURENCE J ZWIEBEL
金额：
$ 56.92万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-06 至 2020-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9552301
关键词：
Acetophenones Acids Address Adult Affect African Anopheles Genus Anopheles gambiae Behavior Behavioral Biological Blood Chemicals Code Collaborations Complex Cues Culicidae Data Data Set Dengue Development Disease Electrophysiology (science)Elements Exhibits Family Female Generations Heart Human Infection Insect Vectors Insecta International Investigation Kairomones Location Malaria Maps Mediating Modeling Molecular Mosquito Control Neurons Odorant Receptors Odors Olfactory Pathways Olfactory Receptor Neurons Oviposition Physiological Physiology Plasmodium falciparum Play Process Protocols documentation Role Salivary Glands Signal Pathway Signal Transduction Site Smell Perception Specificity Sporozoites System West Nile Encephalitis Yellow Fever Zika Virus appendage base behavioral response combinatorial comparative design disease transmission feeding human disease in vivo insight malaria infection novel novel strategies pathogen preference programs protein expression protein function receptor response sensory input transcriptome transcriptome sequencing transmission process vector vector mosquito vector transmission

项目摘要

Project Summary/Abstract Chemosensory responses are critical components in the control of several essential behaviors of insects that are vectors for pathogens responsible for many important human diseases. In particular, olfaction plays a major role in host seeking and oviposition preference/selection behaviors of blood-feeding female mosquitoes and, as such, constitutes a critical component of the mosquito's ability to transmit diseases such as malaria, dengue, yellow fever, Zika and West Nile virus encephalitis. Within this context, and together with our colleagues, we have undertaken a molecular, cellular and organismal examination of several elements of the olfactory signal transduction cascade in the principal African malaria vector mosquitoes Anopheles gambiae, An. coluzzii, An. arabiensis and An. funestus. An increased understanding of olfactory mechanisms and their underlying chemical cues provides insight into the processes of insect behavioral responses in general and disease transmission in particular and would likely be instrumental in the development of novel mosquito control strategies. In this context we have made significant progress in the characterization of several aspects of olfactory process in mosquito systems leading to, among other things, the identification and functional characterization of a family of odorant receptor proteins that lie at the heart of the olfactory signaling pathway. Building on those advances, this proposal for competitive renewal focuses on extending several elements of our ongoing program including characterization of: (1) the in vivo impact of biologically relevant Plasmodium falciparum infections on OR expression and OR-based olfactory receptivity, (2) the olfactory physiology and functional role of labellum ORs in An. coluzzii and (3) an examination of anthropophily via a comparative analysis of OR expression and functionality across Anophelines.

项目总结/摘要化学感觉反应是控制神经元的几种基本行为的关键组成部分。昆虫是导致许多重要人类疾病的病原体的载体。尤其是嗅觉在吸血雌虫的寄主寻找和产卵偏好/选择行为中起主要作用因此，它构成了蚊子传播疾病的能力的关键组成部分，如疟疾、登革热、黄热病、寨卡病毒和西尼罗河病毒脑炎。在此背景下，我们的同事，我们已经进行了分子，细胞和有机体检查的几个要素，非洲疟疾主要媒介蚊的嗅觉信号传导级联 gambiae、冈比亚角隐翅虫An. coluzzii，安. arabiensis和安. funestus。增加对嗅觉机制的了解其潜在的化学线索提供了深入了解昆虫行为反应的过程，一般和疾病的传播，特别是可能有助于发展新的灭蚊策略。在这方面，我们在几个方面取得了重大进展，蚊子系统中的嗅觉过程的各个方面，除其他外，嗅觉信号核心的气味受体蛋白家族的功能表征通路在这些进展的基础上，这项竞争性续约建议的重点是延长几个我们正在进行的计划的要素，包括表征：（1）生物相关的体内影响，恶性疟原虫感染对OR表达和基于OR的嗅觉感受性的影响，（2）嗅觉感受性唇区ORs生理和功能作用coluzzii和（3）通过一个跨按蚊属的OR表达和功能的比较分析。