Molecular Characterization of an Anopheline Ammonium Transporter

按蚊铵转运蛋白的分子表征

基本信息

批准号：
9278096
负责人：
LAURENCE J ZWIEBEL
金额：
$ 19.61万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-06-01 至 2019-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9278096
关键词：
Address Adult Ammonia Ammonium Anopheles gambiae Attention Behavior Behavioral Bite Blood Carrier Proteins Chemicals Clustered Regularly Interspaced Short Palindromic Repeats Comparative Study Culicidae Development Drosophila genus Evolution Female Gene Family Gene Silencing Gene Targeting Generations Goals Human Immune Sera Insecta Investigation Kairomones Lead Life Light Location Malaria Maps Maxilla Mediating Messenger RNA Molecular Morbidity - disease rate Mosquito-borne infectious disease Odorant Receptors Oviposition Pathway interactions Phenotype Physiological Play Process Protein Family Proteins RNA Interference Research Resources Role Sensory Series Signal Transduction Site Smell Perception Supporting Cell Variant appendage base cell type design disorder control experimental study feeding in vivo innovation insight knockout gene malaria transmission member mortality mutant novel novel strategies null mutation preference receptor response sensory input transmission process vector vector mosquito virtual

项目摘要

Project Summary The ability of the malaria vector mosquito Anopheles gambiae to sense and locate human blood meal hosts remains rudimentary. In this process, chemosensory signals and olfaction in particular, provide the dominant sensory inputs for Anopheline and other mosquitoes and accordingly play a critical role in modulating behaviors that impact vectorial capacity. The goal of this revised proposal is to extend ongoing preliminary studies to characterize the molecular pathways underlying the mosquito's ability to sense and respond to ammonia that is an essential component of human-derived odorants that attract blood-feeding females. Here we focus on the role of AgAmt1, a putative ammonia transporter in Anopheles gambiae that may be required for the olfactory sensory transduction of ammonia, which is a critically important, human-derived kairomone for mosquito host seeking. These studies will provide additional insights into the mechanisms of insect olfaction and constitute an important resource for the design and implementation of effective disease control strategies that target Anopheles gambiae and a wide range of related vector species.

项目摘要疟疾矢量蚊子蚊子冈比亚的能力感知和定位人类血液粉主人仍然是基本的。在此过程中，尤其是化学感应信号和嗅觉，提供了截肢和其他蚊子的主要感觉输入，因此在调节中起着关键作用影响矢量能力的行为。该修订的建议的目的是扩展持续的初步研究表征蚊子感知和反应能力的分子途径的研究氨是吸引血液喂食雌性的人类衍生气味的重要组成部分。这里我们专注于Agamt1的作用，Agamt1是一种可能需要的冈比亚河流中推定的氨转运蛋白对于氨的嗅觉转导氨，这是一个至关重要的人类衍生的Kairomone 蚊子主持人寻求。这些研究将提供有关昆虫嗅觉机制的更多见解并构成设计和实施有效疾病控制策略的重要资源该靶向冈比亚山脉和广泛的相关载体物种。