A Melamine Field Sensor for Surety Control of Dairy Products

用于乳制品安全控制的三聚氰胺现场传感器

• 批准号: 7907432
• 负责人: KEVIN M SPENCER
• 金额: $ 9万
• 依托单位: EIC LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2011-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7907432
• 关键词: Melamine; Field; Sensor; Surety; Control

DESCRIPTION (provided by applicant): An inexpensive disposable sensing element that can detect melamine contamination in dairy products and serve as an on-site quality control monitor is proposed. This sensor, coupled to a battery operated reader, will serve a specific surety control needed in the marketplace after the China milk contamination of 2008. The sensor is based on Surface-Enhanced Raman Spectroscopy (SERS), which allows precise detection of chemicals that adsorb strongly to roughened SERS sensors, like melamine, into the ppb range. The SERS sensors, vapor deposited silver films with subsequent electrochemical roughening, are inexpensive to make and can be used in complex matrices like milk. As the sensors are tuned to the analytes of interest, interferences from more concentrated chemicals is limited. Therefore, an extensive separation of milk components is not required. It will be shown that a simple one-step protein denaturation is the only preprocessing required before measurement. In this program, a simple protocol suitable for on-site applications wherein a milk sample is drawn, rapidly denatured, the aqueous phase sampled and the SERS measurement made in 5 minutes or less will be presented. The SERS sensors will be read using a battery operated Raman instrument with keyed sampling chamber that ensures the SERS sensing elements are automatically aligned into the system. The compact system would be fully automated and incorporate a spectral library with partial least squares analysis. Identification and quantification of melamine would be reported to the field technician. A single reader can be used for multiple field site evaluations. The portable test would complement more sophisticated laboratory methods and would prevent tainted milk from reaching the consumer. The core concepts of this technology have been developed and demonstrated. Literature results show SERS detection of melamine and EIC Laboratories has preliminary evidence for detection of melamine in whole milk. The Phase I program is designed to define optimize and quantify 1) the SERS sensors to be utilized in this application and 2) the protein denaturing step. The latter will be optimized for melamine yield in the aqueous phase while the former will be optimized for durability, sensitivity and reproducibility. The Phase I program will demonstrate detection limits, signal linearity and precision for the detection of melamine in whole milk, 2% milk, fat-free milk and heavy cream. In the Phase I program, it will be demonstrated that variances in the milk matrix will not affect the SERS measurement precision for melamine. PUBLIC HEALTH RELEVANCE: Relevance: The proposed portable SERS reader with disposable sensing elements that can be used to monitor melamine contamination of milk gives the FDA and other regulatory agencies a rapid reproducible detection method to use at key manufacturing facilities without waiting for laboratory analyses. The sensors will allow larger data samplings at lower costs and can be extended to other potential contaminants.

描述（由申请人提供）：提出了一种廉价的一次性传感元件，其可以检测乳制品中的三聚氰胺污染并用作现场质量控制监测器。该传感器与电池驱动的阅读器相连，将在2008年中国牛奶污染事件后为市场提供所需的特定监控。该传感器是基于表面增强拉曼光谱（Sers），它可以精确检测化学品的强烈吸附粗糙的Sers传感器，如三聚氰胺，到ppb的范围。Sers传感器，气相沉积银膜，随后进行电化学粗糙化，制造成本低廉，可用于牛奶等复杂基质。由于传感器被调谐到感兴趣的分析物，来自更浓缩的化学品的干扰是有限的。因此，不需要对乳组分进行大量分离。它将表明，一个简单的一步蛋白质变性是测量前所需的唯一预处理。在这个程序中，一个简单的协议适用于现场应用，其中牛奶样品被抽取，快速变性，水相取样和Sers测量在5分钟或更短的时间内进行。Sers传感器将使用电池供电的拉曼仪器读取，该仪器具有键控采样室，以确保Sers传感元件自动对准系统。该紧凑型系统将是全自动的，并将光谱库与偏最小二乘分析相结合。三聚氰胺的鉴定和定量将报告给现场技术人员。一个读数器可用于多个现场评估。便携式检测将补充更复杂的实验室方法，并将防止污染牛奶到达消费者手中。这项技术的核心概念已经开发和论证。文献结果表明Sers检测三聚氰胺和EIC实验室有初步证据检测全脂牛奶中的三聚氰胺。第一阶段计划旨在定义、优化和量化1）本应用中使用的Sers传感器和2）蛋白质变性步骤。后者将针对水相中的三聚氰胺产率进行优化，而前者将针对耐久性、灵敏度和再现性进行优化。第一阶段计划将展示检测全脂牛奶、2%牛奶、脱脂牛奶和重奶油中三聚氰胺的检测限、信号线性和精密度。在第一阶段计划中，将证明牛奶基质的变化不会影响三聚氰胺的Sers测量精度。 公共卫生关系：相关性：拟议中的便携式Sers读取器带有一次性传感元件，可用于监测牛奶中的三聚氰胺污染，为FDA和其他监管机构提供了一种快速可重复的检测方法，可用于关键的生产设施，而无需等待实验室分析。传感器将允许以较低的成本进行更大的数据采样，并可以扩展到其他潜在的污染物。