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Rapid Pesticide Exposure Analysis Using Surface-Enhanced Raman Spectroscopy

使用表面增强拉曼光谱进行快速农药暴露分析

基本信息

批准号：
7496169
负责人：
KEVIN M SPENCER
金额：
$ 29.96万
依托单位：
EIC LABORATORIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-15 至 2010-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7496169
关键词：
Adsorption Affect Agriculture Air Algorithms Be++ element Beryllium Binding Biological Biology Centers for Disease Control and Prevention (U.S.)Chemical Exposure Chemicals Chlorinated Hydrocarbons Class Communication Complex Condition Coupled Creatinine Daily Data Deposition Detection Development Elements Ensure Environmental Exposure Environmental Monitoring Evaluation Exposure to Film Fingerprint Goals Gold Herbicides Human Humidity Industrial fungicide Ingestion Laboratories Laboratory Procedures Lasers Left Libraries Liquid substance Literature Measurement Measures Metals Methods Monitor Organophosphates Ownership Participant Pattern Recognition Pesticide Residues Pesticides Phase Positioning Attribute Process Raman Spectrum Analysis Range Reader Reading Reporting Research Sampling Signaling Molecule Solutions Specificity Spectrum Analysis Standards of Weights and Measures Structure Surface System Techniques Technology Time United States National Institutes of Health Universities Urinalysis Urine Wireless Technology air contaminant aqueous base concept design detector farm worker forest innovation instrument interest medical schools miniaturize nano organochlorine pesticide pesticide exposure photonics planetary Atmosphere programs sensor tool vapor

项目摘要

DESCRIPTION (provided by applicant) An inexpensive disposable sensing element that is worn as a vapor exposure monitor or used for urinalysis is proposed for real-time monitoring of pesticide exposure. This sensor, coupled to a battery operated reader, is a direct need for the NIH Exposure Biology Program and has the potential for detect other exposures as well. The sensor is based on Surface-Enhanced Raman Spectroscopy (SERS), which allows precise detection of chemicals that adsorb strongly to roughened SERS sensors, like pesticides, into the high ppt range. The SERS sensors, vapor deposited gold films with subsequent electrochemical roughening, are inexpensive to make, operate in vapor phase under a wide humidity range and in solution. As the sensors are tuned to the analytes of interest, interferences from more concentrated chemicals is limited. In this program, the SERS sensors would be worn as badges to monitor and quantify daily pesticide exposure to a worker by irreversibly binding any pesticide that comes in contact with the SERS sensor. The sensors would also measure pesticide metabolites in urine, providing the NIH with more data on the daily exposure/daily ingestion of pesticides. The SERS sensors will be read using a battery operated Raman instrument with keyed sampling chamber that ensures the SERS sensing elements are automatically aligned into the system. The compact system would be fully automated and incorporate a spectral library with chemometric analysis. Identification and quantification of any detected pesticides would be reported to the field technician. A single reader can be used for evaluation of hundreds of participants. The core concepts of this technology have been developed and demonstrated. Literature results show SERS detection of organochlorine pesticides and EIC Laboratories has detected organophosphate pesticides in vapor and aqueous phases. Field detection of trace levels of explosives has been demonstrated by EIC Laboratories. The two year Phase I program is designed to expand the pesticides studied, demonstrate detection to CDC listed limits, show an adequate usable field lifetime for the SERS sensing elements, design a fieldable reader, perform a detailed interferences analysis and demonstrate that variances in the urine matrix will not affect the SERS measurement precision. Commercial: EIC Laboratories will commercialize the technology by manufacturing the SERS sensors and battery operated Raman readers. The components will be sold commercially through In Photonics, an affiliated company under common ownership.

描述（由申请人提供）建议使用一种廉价的一次性传感元件作为蒸汽暴露监测器或用于尿液分析来实时监测农药暴露。该传感器与电池供电的读取器相结合，是 NIH 暴露生物学计划的直接需求，并且还具有检测其他暴露的潜力。该传感器基于表面增强拉曼光谱 (SERS)，可精确检测高 ppt 范围内强烈吸附于粗糙 SERS 传感器的化学物质（如杀虫剂）。 SERS 传感器采用气相沉积金膜并随后进行电化学粗糙化，制造成本低廉，可在宽湿度范围下的气相和溶液中工作。当传感器针对感兴趣的分析物进行调整时，来自更浓缩化学物质的干扰受到限制。在此计划中，SERS 传感器将作为徽章佩戴，通过不可逆地结合与 SERS 传感器接触的任何农药，来监测和量化工人每天接触的农药。这些传感器还将测量尿液中的农药代谢物，为美国国立卫生研究院提供有关每日接触/每日摄入农药的更多数据。 SERS 传感器将使用带有键控采样室的电池供电拉曼仪器进行读取，以确保 SERS 传感元件自动对齐到系统中。这个紧凑的系统将是完全自动化的，并包含一个具有化学计量分析功能的光谱库。任何检测到的农药的鉴定和定量将报告给现场技术人员。单个阅读器可用于评估数百名参与者。该技术的核心概念已经得到开发和论证。文献结果表明，SERS检测有机氯农药，EIC实验室已检测到气相和水相中的有机磷农药。 EIC 实验室已经证明了痕量爆炸物的现场检测。为期两年的第一阶段计划旨在扩大研究的农药，证明检测达到 CDC 列出的限值，显示 SERS 传感元件足够的可用现场寿命，设计可现场读取器，进行详细的干扰分析，并证明尿液基质的变化不会影响 SERS 测量精度。商业化：EIC 实验室将通过制造 SERS 传感器和电池供电的拉曼读取器来将该技术商业化。这些组件将通过 In Photonics（一家共同拥有的附属公司）进行商业销售。