Monitoring Pesticide Exposure and Accumulation: An improved Rapid Identifier for all Pesticide Classes

 描述（由申请人提供）：一种廉价的一次性蒸汽传感器，也可用于尿液分析，可提供农药暴露的快速现场监测。该传感器利用表面增强拉曼光谱（Sers）检测化学品，如农药，强烈吸附在粗糙的Sers基板在高部分前万亿范围。由于传感器被调谐到感兴趣的分析物，来自更浓缩的化学品的干扰是有限的。早期的研究计划为有机磷（OP）和有机氯（OC）农药提供了可靠的结果。在该方案中，在实验室中对50种OP和OC农药或代谢物进行了低ppb范围的评估，并在农场工人营地进行了乙酰甲胺磷和甲基敌百虫检测。在本计画中，我们将制作一种新颖的Sers感测元件，以侦测三嗪类农药，如阿特拉津、西玛津及灭蝇胺。传感器将通过叠氮化物负载来优化，以提高电子密度并产生与三嗪胺基团的强氢键。这种新型传感器预计将检测浓度为1 ppb或更低的三嗪，并承受农场工人遇到的温度和湿度条件。新的传感器可以很容易地与我们以前的传感器相结合，以当前分析实验室成本的一小部分测量>75种农药或代谢物。 方法，从而为NIH（特别是NIEHS暴露生物学计划）提供了大量关于农药每日暴露/摄入的数据集。这些数据将有助于进行流行病学研究和预测长期健康结果。核心技术概念已得到验证。以前的一个项目表明，有机氯和有机磷农药可以检测到低至100 ppt。EIC实验室在初步研究中证明，叠氮化物涂层的Sers传感器对三嗪的敏感性比以前的传感器高几个数量级。第一阶段计划旨在证明在1 ppb水平上检测三嗪农药和代谢物，显示延长的现场寿命和增加的三嗪农药代谢物直接读数的灵敏度。

 DESCRIPTION (provided by applicant): An inexpensive disposable vapor sensor, which can also be used for urinalysis, can provide rapid field monitoring of pesticide exposure. The sensor utilizes Surface-Enhanced Raman Spectroscopy (SERS) to detect chemicals, like pesticides, that adsorb strongly on roughened SERS substrates in the high parts-pre- trillion range. As the sensors are tuned to the analytes of interest, interferences from more concentrated chemicals are limited. An earlier research program provided reliable results for organophosphate (OP) and organochlorine (OC) pesticides. In that program, 50 OP and OC pesticides or metabolites were evaluated in the laboratory at the low ppb range, with acephate and methyl parathion detection at farmworker camps. In this program, we will fabricate an innovative SERS sensing element for the detection of triazine pesticides, such as atrazine, simazine and cyromazine. Sensors will be optimized by an azide loading to improve the electron density and yield strong hydrogen bonding with the triazine amine groups. This novel sensor is expected to detect triazines in concentrations of 1 ppb or less and withstand the temperature and humidity conditions encountered by farmworkers. The new sensor can be easily combined with our previous sensor to measure >75 pesticides or metabolites at a fraction of the cost of current analytical laboratory methods, thus providing the NIH (particularly the NIEHS Exposure Biology program) with a large data set on the daily exposure/ingestion of pesticides. This data will allow epidemiological studies and predictions of long-term health outcomes. Core technology concepts have been demonstrated. A previous program demonstrated organochlorine and organophosphate pesticides can be detected down as low as 100 ppt. EIC Laboratories has demonstrated in preliminary studies that an azide-coated SERS sensor is several orders of magnitude more sensitive to triazines than previous sensors. The Phase I program is designed to demonstrate detection of triazine pesticides and metabolites at the 1 ppb level, show an extended field lifetime and an increased sensitivity for direct readings of triazine pesticide metabolites.