ABI Elite ESI-QqTOF Mass Spectrometry System

ABI Elite ESI-QqTOF 质谱系统

• 批准号: 7792846
• 负责人: NATALIE G. AHN
• 金额: $ 45.84万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792846
• 关键词: Biological Factors; Biomedical Research; Chemicals; Colorado; Coupled; Deuterium Exchange Measurement; Development; Diagnosis; Funding; Goals; High Pressure Liquid Chromatography; Laboratories; Ligands; Liquid Chromatography; Manufacturer Name; Maps; Mass Spectrum Analysis; Measurement; Nucleic Acids; Organic Chemistry; Polymers; Proteins; Proteomics; Pulsar; Research; Research Personnel; Research Support; Resolution; Sampling; Scanning; Services; Spectrometry, Mass, Electrospray Ionization; System; Technology; Time; Universities; Water; human disease; instrument; ionization; mass spectrometer; protein aminoacid sequence; protein complex; public health relevance; repaired; small molecule; synthetic nucleotide; ultra high pressure

DESCRIPTION (provided by applicant): We request funds to purchase an integrated system for liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS). The system includes a Applied Biosystems (ABI) QStar Elite quadrupole time-of-flight (QqTOF) mass spectrometer capable of rapid scanning and high accuracy mass measurements, and a Waters ultra-high pressure liquid chromatography system for high resolution analyte separations. The proposed instrument will replace a current ABI QStar Pulsar QqTOF mass spectrometer, which must be retired on July 1, 2010, when ABI terminates all repair and service of the current instrument. The system represents a key instrument at the Central Analytical Facility, which serves investigators across the University of Colorado at Boulder (CU-Boulder) campus. The current QqTOF instrument is our workhorse for service samples analyzed by the facility and is currently used by more than 20 laboratories across this campus. It is most heavily used to support research in synthetic organic chemistry and materials development, including structural and elemental analysis of small molecules, natural products, and polymers, and for negative and positive ionization mode analysis of synthetic nucleotides and nucleic acids. It is also used extensively for proteomics applications, including hydrogen-deuterium exchange measurements to map protein-protein and protein-ligand interfaces, and routine ESI-LC-MS/MS to carry out peptide sequencing of protein digests in order to determine subunit compositions of protein complexes. The current QqTOF instrument is used nearly 24/7, and is an essential component of the Central Analytical Facility. Its replacement with the QStar Elite QqTOF is critically needed to continue supporting a wide range of research needs at CU-Boulder. PUBLIC HEALTH RELEVANCE: Funding is requested to purchase a new mass spectrometry system, to replace an existing system that will be retired in 2010, when the manufacturer discontinues repair and service. This is a key analytical technology for "weighing" molecules, which is essential for biomedical research because it allows the chemical composition of molecules to be defined. The instrument will be shared by more than 20 laboratories at the University of Colorado at Boulder, who are synthesizing new materials or characterizing proteins, with the goal of understanding how to diagnose and treat human diseases.

描述(由申请者提供)：我们申请资金购买一个用于液相色谱和电喷雾电离质谱仪(LC-ESI-MS)的集成系统。该系统包括能够快速扫描和高精度质量测量的应用生物系统(ABI)QStar Elite四极杆飞行时间(QqTOF)质谱仪，以及用于高分辨率分析物分离的Waters超高压液相色谱系统。拟议中的仪器将取代目前ABI QStar Pulsar QqTOF质谱仪，后者必须在2010年7月1日退役，届时ABI将终止对当前仪器的所有维修和服务。该系统代表着中央分析设施的一个关键仪器，该设施为科罗拉多大学博尔德分校(CU-Boulder)的调查人员提供服务。目前的QqTOF仪器是我们通过设施分析服务样品的主力，目前被整个园区的20多个实验室使用。它主要用于支持合成有机化学和材料开发的研究，包括小分子、天然产物和聚合物的结构和元素分析，以及合成核苷酸和核酸的正负电离模式分析。它还被广泛用于蛋白质组学应用，包括氢-重离子交换测量以绘制蛋白质-蛋白质和蛋白质-配体界面，以及常规的ESI-LC-MS/MS对蛋白质消化进行肽序列测定，以确定蛋白质复合体的亚单位组成。目前的QqTOF仪器几乎全天候使用，是中央分析设施的一个重要组成部分。为了继续支持CU-Boulder的广泛研究需求，迫切需要用QStar Elite QqTOF来取代它。 与公共健康相关：申请资金购买新的质谱分析系统，以取代将于2010年退役的现有系统，届时制造商将停止维修和服务。这是一项关键的分子称重分析技术，这对生物医学研究至关重要，因为它允许定义分子的化学组成。科罗拉多大学博尔德分校的20多个实验室将共享该仪器，这些实验室正在合成新材料或表征蛋白质，目的是了解如何诊断和治疗人类疾病。

项目成果

Modular Synthesis of Rigid Polyacene Dimers for Singlet Fission

• DOI: 10.1021/acs.orglett.7b03817
• 发表时间: 2018-01-19
• 期刊: ORGANIC LETTERS
• 影响因子: 5.2
• 作者: Carey, Thomas J.;Miller, Ethan G.;Damrauer, Niels H.
• 通讯作者: Damrauer, Niels H.

Glycosyl Cross-Coupling of Anomeric Nucleophiles: Scope, Mechanism, and Applications in the Synthesis of Aryl C-Glycosides.

• DOI: 10.1021/jacs.7b08707
• 发表时间: 2017-12-13
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Zhu F;Rodriguez J;Yang T;Kevlishvili I;Miller E;Yi D;O'Neill S;Rourke MJ;Liu P;Walczak MA
• 通讯作者: Walczak MA

The Partitioning of Small Aromatic Molecules to Air-Water and Phospholipid Interfaces Mediated by Non-Hydrophobic Interactions.

• DOI: 10.1021/acs.jpcb.6b05084
• 发表时间: 2016-07
• 期刊: The journal of physical chemistry. B
• 作者: Russell J. Perkins;A. Kukharchuk;P. Delcroix;R. Shoemaker;Martina Roeselová;Lukasz Cwiklik;V. Vaida

Stereoselective oxidative glycosylation of anomeric nucleophiles with alcohols and carboxylic acids.

• DOI: 10.1038/s41467-018-06016-4
• 发表时间: 2018-09-07
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Yang T;Zhu F;Walczak MA
• 通讯作者: Walczak MA

Facile accelerated specific therapeutic (FAST) platform develops antisense therapies to counter multidrug-resistant bacteria.

• DOI: 10.1038/s42003-021-01856-1
• 发表时间: 2021-03-12
• 期刊: Communications biology
• 影响因子: 5.9
• 作者: Eller KA;Aunins TR;Courtney CM;Campos JK;Otoupal PB;Erickson KE;Madinger NE;Chatterjee A
• 通讯作者: Chatterjee A