Leica TCS SP5 Spectral LSCM for the Research Imaging Facility

用于研究成像设施的 Leica TCS SP5 光谱 LSCM

• 批准号: 7793952
• 负责人: Teresa Liberati
• 金额: $ 50万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY SCH OF MED
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793952
• 关键词: Affect; Age; Area; Cells; Communicable Diseases; Computer software; Core Facility; Data Collection; Experimental Designs; Faculty; Funding; Health; Human; Illinois; Image; Journals; Lasers; Malignant Neoplasms; Manuscripts; Microscope; Pathway interactions; Peer Review; Photobleaching; Phototoxicity; Process; Productivity; Publications; Research; Research Personnel; Resolution; Scanning; Signal Transduction; Source; System; Technology; United States National Institutes of Health; Universities; cellular development; flexibility; improved; interest; medical schools; meetings; member; repaired; tool

DESCRIPTION (provided by applicant): The Southern Illinois University School of Medicine (SIUSM) requests support to purchase a new laser scanning confocal microscope for our research imaging core facility. The requested Leica TCS SP5 Laser Scanning Confocal Microscope system will replace an Olympus Fluoview LSCM that was purchased in 1999 by SIUSM and remains in use today as our only confocal microscope. However, this unit is outdated and support from Olympus is not readily available due to the age of the microscope. Obtaining timely repairs is difficult and an impediment to faculty productivity and the microscope no longer meets the research needs of our faculty. The Leica TCS SP5 Laser Scanning Confocal Microscope system will support a core of NIH-funded faculty members with diverse research interests as indicated by their varied sources of NIH funding. The Leica microscope will increase the quality and quantity of data collection, available research options, research productivity and quality of manuscripts submitted for publication in peer-reviewed journals. Compared to our existing microscope, the new Leica system offers: 1) improved sensitivity by reducing signal loss in both the excitation and emission pathways; 2) improved resolution; 3) reduced photobleaching and phototoxicity; 4) greater flexibility in experimental design with multiple laser lines; and 5) enhanced analysis capabilities with improved software applications. This upgrade in technology will contribute to more rapid advances in our understanding of the causes and course of cancer, infectious diseases, cell development, and cellular and biomolecular processes affecting human health. Thus, overall scientific productivity and efficiency will be increased. These benefits will allow our NIH-funded and other investigators to advance their respective areas of research in ways not otherwise possible with the research tools currently available to them at SIUSM.

描述（由申请人提供）：南伊利诺伊大学医学院 (SIUSM) 请求支持为我们的研究成像核心设施购买一台新的激光扫描共焦显微镜。所要求的 Leica TCS SP5 激光扫描共焦显微镜系统将取代 SUSM 于 1999 年购买的奥林巴斯 Fluoview LSCM，至今仍作为我们唯一的共焦显微镜使用。然而，该装置已经过时，并且由于显微镜的年代久远，奥林巴斯的支持也不容易获得。及时进行维修很困难，这会妨碍教师的工作效率，而且显微镜不再满足我们教师的研究需求。 Leica TCS SP5 激光扫描共焦显微镜系统将为 NIH 资助的核心教职人员提供支持，这些教职人员具有不同的研究兴趣，这体现在他们的 NIH 资金来源多种多样。徕卡显微镜将提高数据收集的质量和数量、可用的研究选项、研究生产力以及提交在同行评审期刊上发表的手稿的质量。与我们现有的显微镜相比，新的徕卡系统提供：1）通过减少激发和发射路径中的信号损失来提高灵敏度； 2）提高分辨率； 3）减少光漂白和光毒性； 4）多条激光线的实验设计具有更大的灵活性； 5) 通过改进的软件应用程序增强分析能力。这种技术升级将有助于我们更快地了解癌症、传染病、细胞发育以及影响人类健康的细胞和生物分子过程的原因和病程。因此，整体科学生产力和效率将得到提高。这些好处将使我们的 NIH 资助的研究人员和其他研究人员能够以 SIUSM 目前可用的研究工具无法实现的方式推进各自的研究领域。