Integrated AFM and Real-Time Confocal Microscope Core

集成 AFM 和实时共焦显微镜核心

• 批准号: 7794679
• 负责人: KEVIN D COSTA
• 金额: $ 46.92万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-08 至 2012-07-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794679
• 关键词: Arts; Biological; Biophysics; Cardiovascular system; Cells; Confocal Microscopy; Consultations; Core Facility; Engineering; Ensure; Environment; Funding; Generations; Grant; Hand; Imagination; Imaging Device; Individual; Institutes; Institution; Investments; Knowledge; Life; Maps; Measurement; Methods; Microscope; Molecular; Names; Nanotechnology; Optical Methods; Optics; Research; Resolution; Sampling; Scanning; Scanning Probe Microscopes; Semiconductors; System; Time; Training; United States National Institutes of Health; aqueous; catalyst; cohort; design; instrument; interdisciplinary collaboration; novel; research study; sensor

DESCRIPTION (provided by applicant): The atomic force microscope (AFM) was originally designed as a high-resolution imaging tool for semiconductor scanning applications to allow measurement of atomic level features and forces. The ability to be used in an aqueous environment also allows interaction with biological molecules and even living cells. A unique feature of the AFM is that it is not an optical method, but rather uses a scanning probe that physically contacts the sample to achieve sub-optical resolution. The high-sensitivity force sensor can be used for quantifying the micromechanics involved in pushing, pulling, and manipulating objects from individual molecules to whole cells, providing a unique mapping of the rich mechanobiology landscape. The latest generation of atomic force microscopes allows seamless integration of AFM and confocal microscopy, combining the unique advantages of each method and ushering the AFM out of engineering and biophysics labs and into the hands of cell and molecular biologists who find new applications for this cutting- edge nanotechnology. This shared instrument grant proposes to acquire a state-of-the-art integrated atomic force and real-time confocal microscope as a departmental core facility in the Cardiovascular Research Center. No other such system has ever existed at Mount Sinai, and the current proposal takes advantage of a unique confluence of opportunity and expertise. With an initial cohort of 8 NIH-funded major users, including MDs and PhDs representing 5 departments and 5 NIH institutes, this instrument will significantly impact ongoing research efforts and provide novel ways to achieve the proposed aims. Dr. Costa's new arrival at Mount Sinai will serve as a catalyst to stimulate the imagination and brainstorm ideas while maintaining a firm understanding of the capabilities and limitations of the AFM. The expertise of the PI and the named technician, in consultation with a diverse local management committee, will ensure proper training of users, and maximal and equitable use of the shared instrument. The resulting interactions will open doors for new experiments and interdisciplinary collaborations that were never before possible at Mount Sinai, helping to expand the boundaries of biomedical knowledge and discovery in research at an institution with a top-20 ranking in total NIH investment.

描述（由申请人提供）：原子力显微镜（AFM）最初被设计为用于半导体扫描应用的高分辨率成像工具，以允许测量原子级特征和力。在水性环境中使用的能力也允许与生物分子甚至活细胞相互作用。AFM的一个独特之处在于它不是一种光学方法，而是使用物理接触样品的扫描探针来实现亚光学分辨率。高灵敏度力传感器可用于量化推、拉和操纵从单个分子到整个细胞的物体所涉及的微观力学，为丰富的机械生物学景观提供独特的映射。最新一代的原子力显微镜允许原子力显微镜和共聚焦显微镜的无缝集成，结合了每种方法的独特优势，并将原子力显微镜带出工程和生物物理实验室，进入细胞和分子生物学家手中，他们为这种尖端的纳米技术找到了新的应用。这项共享仪器补助金提议购买最先进的集成原子力和实时共聚焦显微镜作为心血管研究中心的部门核心设施。西奈山从来没有其他这样的系统，目前的建议利用了独特的机会和专业知识的融合。该工具最初由8名NIH资助的主要用户组成，包括代表5个部门和5个NIH研究所的医学博士和博士，将对正在进行的研究工作产生重大影响，并为实现拟议目标提供新的方法。科斯塔博士在西奈山的新的到来将作为一个催化剂，以刺激想象力和头脑风暴的想法，同时保持对AFM的能力和局限性的坚定理解。PI和指定技术人员的专业知识，在与不同的当地管理委员会协商后，将确保对用户进行适当的培训，并最大限度地公平使用共享仪器。由此产生的互动将为西奈山以前从未有过的新实验和跨学科合作打开大门，有助于扩大NIH总投资排名前20位的机构的生物医学知识和研究发现的边界。