High-throughput Workstation For Cell-based Assays

用于细胞分析的高通量工作站

• 批准号: 7793037
• 负责人: DAN P FELSENFELD
• 金额: $ 48.65万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793037
• 关键词: Biological Assay; Cells; Detection; Developmental Biology; Device or Instrument Development; Fluorescence; Health; Housing; Human; Libraries; Liquid substance; Maintenance; Mental disorders; Methods; Neurology; Process Measure; Psychotic Disorders; Reader; Research Personnel; Research Support; Resource Sharing; Screening procedure; Small Interfering RNA; System; Training Support; base; cancer pain; flu; genome-wide; high throughput screening; instrument; luminescence; medical schools; novel; oncology; virology

DESCRIPTION (provided by applicant): A high-throughput workstation for cell-based assays is requested for the use for researchers at the Mount Sinai School of Medicine. This system is comprised of a liquid handler integrated with a plate reader (with fluorescence, luminescence and absorbance detection) for processing and measuring multiwell assay plates. Users at Mount Sinai will use the instrument for the development and execution of cell-based high-throughput screens of siRNA libraries. This instrument will be housed in and maintained by the High-Content Screening Shared Resource Facility (HCS-SRF), a centralized organization at Mount Sinai that is directed at providing access to genome-wide siRNA screening methods to Mount Sinai researchers. The facility's staff of two dedicated biomedical researchers will provide ongoing support and training for users of the microwell plate-reader system and will be responsible for its maintenance. PROJECT NARRATIVE: The requested instrument will support research in virology, neurology, oncology and developmental biology. The information generated on this system could help to indentify novel therapies for the treatment of a variety of major human health problems, including cancer, pain, flu, and psychiatric disorders, including psychosis.

描述(由申请人提供)：西奈山医学院的研究人员需要一台用于细胞分析的高通量工作站。该系统由液体处理机和平板读取器(具有荧光、发光和吸光度检测)集成，用于处理和测量多孔分析板。西奈山的用户将使用该仪器开发和执行基于细胞的高通量siRNA文库筛选。该仪器将存放在高含量筛选共享资源设施(HCS-SRF)中并由其维护，该设施是西奈山的一个中央组织，旨在为西奈山的研究人员提供获得全基因组siRNA筛选方法的途径。该设施的两名专门的生物医学研究人员将为Microwell平板阅读器系统的用户提供持续的支持和培训，并将负责其维护。 项目简介：申请的仪器将支持病毒学、神经学、肿瘤学和发育生物学的研究。在这个系统上产生的信息可以帮助识别 治疗各种重大人类健康问题的新疗法，包括癌症、疼痛、流感和包括精神病在内的精神疾病。