A TSQ Vantage Mass Spectrometer for the Analytical Chemistry Instrumentation Faci

描述(由申请人提供)：加州大学河滨分校的分析化学仪器(ACIF)正在申请购买LC-MS/MS系统的资金，该系统包括Thermo Fisher Science TSQ Vantage三四极杆质谱仪和Agilent 1200毛细管高效液相色谱系统。所要求的仪器将安装在ACIF，并由加州大学河滨分校的ACIF工作人员管理。在这方面，ACIF的质谱学设施已经为整个河滨校区和全国各地的许多调查人员服务了超过25年。所要求的仪器将主要用于支持加州大学河滨分校NIH资助的一些调查人员正在进行的研究。尽管ACIF拥有几台质谱仪，个别研究人员的实验室中也有其他几台仪器，但加州大学河滨分校没有一台仪器可以满足与拟议仪器相关的研究项目的苛刻分析需求。特别是，缺乏准确量化从复杂生物基质中分离出来的痕量核酸及其成分的能力。此外，目前用户无法在任何仪器上进行真正的前驱离子扫描和中性损失扫描。这两个特征在建议的三重四极质谱计上可用，将对发现新的DNA损伤产物非常有用。所要求的仪器将大大提高参与人员检测和量化受损或修饰的核苷、核苷酸和核酸的能力。 公共卫生相关性：拟议的LC-MS/MS系统将有利于一组由NIH资助的研究人员的研究项目，他们对与核苷、核苷酸和核酸相关的研究感兴趣。这些研究项目的成果将提高我们对包括癌症和糖尿病在内的许多人类疾病的了解。

DESCRIPTION (provided by applicant): The Analytical Chemistry Instrumentation Facility (ACIF) at the University of California Riverside is requesting funds for purchasing an LC-MS/MS system, which includes a Thermo Fisher Scientific TSQ Vantage triple-quadrupole mass spectrometer and an Agilent 1200 capillary HPLC system. The requested instrument will be installed at ACIF and managed by the ACIF staff at the University of California Riverside. In this respect, the Mass Spectrometry Facility at ACIF has served the whole Riverside campus and many investigators around the nation for more than 25 years. The requested instrument will be used primarily for supporting the ongoing research of a number of NIH-funded investigators at the University of California Riverside. Although ACIF houses several mass spectrometers and there are several other instruments in individual research faculty's labs, there is no instrument on UC Riverside campus that can meet the demanding analytical needs of the research projects associated with the proposed instrument. In particular, there is a lack of capability in quantifying accurately trace amounts of nucleic acids and their constituents isolated from complex biological matrices. In addition, true precursor-ion and neutral-loss scans are not available on any instruments that are presently available to the users. These two features are available on the proposed triple quadrupole mass spectrometer and will be very useful for the discovery of new damage products of DNA. The requested instrument will enhance substantially the capability of the participating faculty in detecting and quantifying damaged or modified nucleosides, nucleotides, and nucleic acids. PUBLIC HEALTH RELEVANCE: The proposed LC-MS/MS system will benefit the research projects of a group of NIH-funded investigators who are interested in research associated with nucleosides, nucleotides, and nucleic acids. The outcome of these research projects will improve our understanding of a number of human diseases including cancer and diabetes.