LTQ Orbitrap XL ETD Hybrid FT High Resolution Mass Spectrometer

LTQ Orbitrap XL ETD 混合 FT 高分辨率质谱仪

• 批准号: 7793941
• 负责人: DAVID L HACHEY
• 金额: $ 47.93万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-25 至 2011-03-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793941
• 关键词: Accounting; Communities; Complex Mixtures; Core Facility; DNA-protein crosslink; Dissociation; Electron Transport; Engineering; Equipment; Faculty; Fees; Funding; Hybrids; Label; Liquid substance; Maintenance; Mass Spectrum Analysis; Minor; Peptides; Performance; Physicians; Post-Translational Protein Processing; Principal Investigator; Procedures; Proteins; Proteomics; Proteomics Shared Resource; Reagent; Research; Research Personnel; Resolution; Resource Sharing; Scientist; Services; Tracer; United States National Institutes of Health; Universities; Work; adduct; base; experience; instrument; instrumentation; mass spectrometer; member; nano; operation; public health relevance; research study

DESCRIPTION (provided by applicant): Proposed here is the purchase of a Thermo Scientific LTQ Orbitrap XL ETD hybrid mass spectrometer equipped with both high-energy collisional dissociation (HCD) and electron transfer dissociation (ETD) capabilities. The instrument will employ an Eksigent Nano Ultra 2D liquid chromatograph for high resolution nanoflow LC separation of complex mixtures of peptides that was recently purchased by the Proteomics Shared Resource. This instrument will be used to support a diverse group of seven major and four minor users that include physician scientists and basic scientists working in five departments, all of whom are extensively NIH-funded. These users were selected from a base of more than 160 principal investigators at Vanderbilt University who have used the Proteomics Shared Resource over the past three years and selected based on their more demanding need for the level of performance afforded by this class of instrumentation. The LTQ Orbitrap XL ETD will be located in the Proteomics Shared Resource, which is one of three centralized MS core facilities operated by the Vanderbilt University Mass Spectrometry Research Center. The instrument will be managed by a team of five professionals in the proteomics core, including the instrument operator and a mass spectrometry engineer, all of whom have specific experience with operation and maintenance of the Orbitrap. Operational funds will come from user fees assessed to investigators and will employ the standard accounting and billing procedures that have been used by the MSRC service cores for several years. An advisory panel will consist of three senior investigators directly representing the users, an ex officio member of Vanderbilt's Office of Research and an independent senior faculty member. The primary uses for ETD on this instrument will be characterization of posttranslational modifications of proteins, studies of protein-DNA crosslinks and identification of protein adducts of reactive metabolites. In addition, the HCD capabilities will enable quantitative proteomics studies using iTRAQ isobaric tagging reagents and other forms of stable isotopic tracer studies, such as SILAC labeling. PUBLIC HEALTH RELEVANCE: The proteomics research community at Vanderbilt University is extensively funded by NIH. More than 160 investigator groups at Vanderbilt University have used the Proteomics Shared Resource since 2006. The Thermo Scientific LTQ Orbitrap XL ETD hybrid mass spectrometer proposed here will bring important new analytical capabilities for characterization of modified proteins and protein adducts that are unavailable with the current equipment in the Shared Resource. Furthermore, this instrument will enable investigators to undertake new experiments, such as quantitative proteomics using iTRAQ reagents that were hitherto impractical with the current instrumentation.

描述（由申请人提供）：这里提议购买一台Thermo Scientific LTQ Orbitrap XL ETD混合质谱仪，该质谱仪配备了高能碰撞离解（HCD）和电子转移离解（ETD）功能。该仪器将采用Eksigent Nano Ultra 2D液相色谱仪，用于高分辨率纳米流LC分离复杂的多肽混合物，该多肽混合物最近由蛋白质组学共享资源公司购买。该仪器将用于支持由7个主要用户和4个次要用户组成的多样化群体，其中包括在5个部门工作的内科科学家和基础科学家，所有这些人都得到了美国国立卫生研究院的广泛资助。这些用户是从范德比尔特大学160多名在过去三年中使用过蛋白质组学共享资源的主要研究人员中挑选出来的，并根据他们对这类仪器所提供的性能水平的更高要求进行选择。LTQ Orbitrap XL ETD将位于蛋白质组学共享资源中，该资源是范德比尔特大学质谱研究中心运营的三个集中MS核心设施之一。该仪器将由蛋白质组学核心的五名专业人员组成的团队管理，包括仪器操作员和质谱工程师，他们都具有操作和维护Orbitrap的具体经验。运营资金将来自向调查人员评估的用户费用，并将采用MSRC服务核心多年来一直使用的标准会计和计费程序。咨询小组将由三名直接代表用户的高级调查员、范德比尔特大学研究办公室的一名当然成员和一名独立的高级教员组成。该仪器上的ETD的主要用途将是蛋白质翻译后修饰的表征，蛋白质- dna交联的研究和活性代谢物的蛋白质加合物的鉴定。此外，HCD功能将使定量蛋白质组学研究能够使用iTRAQ等压标记试剂和其他形式的稳定同位素示踪剂研究，如SILAC标记。