Biomolecular and Proteomics Shared Resource

生物分子和蛋白质组学共享资源(BPSR)的使命是提供具有成本效益的、 范德比尔特癌症研究人员的最先进的仪器和分析专业知识 中心(VICC)。共享的资源由三个专门的实验室组成，专门分析小分子物质 组织和生理液体中的分子(即药物、药物代谢物和内源性代谢物)；a 用于鉴定、表征和定量蛋白质的综合分析蛋白质组学设施； 以及质谱学成像和组织学指导的蛋白质图谱设备。在上一次的时候 竞争更新，这些活动被分成三个独立的共享资源，每个资源 获得“杰出”评级。将它们合并到一个单一的管理结构中已有所改进 效率和服务，同时削减成本。该设施目前拥有17台质谱仪。其他 还提供用于成像和蛋白质组学研究的专门资本设备，如高分辨率 用于整个动物成像研究的冷冻微型机，高分辨率数字显微镜 记录组织学数据，2D凝胶荧光成像和2D凝胶处理机器人。生物信息学 支持人员开发新的软件工具来支持共享资源和维护计算 基础设施。将这一共享资源纳入CCSG提案的理由包括：(1) VICC研究界广泛使用质谱学服务，(2)独特的和 向调查人员提供的一系列全面服务，以及(3)这些服务的成本效益 由一支技术精湛的专业员工组成。在2008财年，95个VICC/CCSG研究小组使用了这些服务。这个 共享资源提供16类分析服务，涵盖范围广泛的分析 用于PK/PD研究的药物定量测量.鉴定、表征和定量 癌症生物标志物；以及对人体活检样本中的蛋白质、脂肪和药物进行成像和分析。这个 共享资源得到CCSG的支持，通过对个人调查人员的研究资助，以及由重要的 来自范德比尔特的制度支持。

The mission of the Biomolecular & Proteomics Shared Resource (BPSR) is to provide cost-effective, state-of-the-art instrumentation and analytical expertise to investigators in the Vanderbilt-lngram Cancer Center (VICC). The shared resource consists of three specialized laboratories dedicated to analysis of small molecules (i.e., drugs, drug metabolites and endogenous metabolites) In tissues and physiologic fluids; a comprehensive analytical proteomics facility for identification, characterization and quantitation of proteins; and a mass spectrometry imaging and histology-directed protein profiling facility. At the time of the last competitive renewal, these activities were separated into three independent shared resources, each receiving an "Outstanding" rating. Combining them into a single administrative structure has improved efficiency and service, while cutting costs. The facility currently houses 17 mass spectrometers. Other specialized capital equipment for imaging and proteomic studies are also available, such as high resolution matrix spotters, a cryomacrotome for whole animal imaging studies, a high resolution digital microscope for documenting histology data, a 2D gel fluorescence imaging and 2D gel processing robot. A bioinformatics support staff develops new software tools to support the shared resource and maintains the computing infrastructure. Justifications for including this shared resource in the CCSG proposal include (1) the extensive use of mass spectrometry services by the VICC research community, (2) the unique and comprehensive array of services offered to investigators, and (3) the cost-effective delivery of these services by a highly skilled professional staff. In FY 2008, 95 VICC/CCSG research groups used these services. The shared resource provides 16 categories of analytical services covering a wide range of analytical measurements from drug quantitation for PK/PD studies; identification, characterization and quantitation of cancer biomarkers; and imaging and profiling of proteins, lipids and drugs in human biopsy samples. The shared resource is supported by the CCSG, by research grants to individual investigators, and by significant institutional support from Vanderbilt.