Biomolecular and Proteomics Shared Resource

生物分子和蛋白质组学共享资源

• 批准号: 8733538
• 负责人: DAVID L HACHEY
• 金额: $ 28.33万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8733538
• 关键词: Applications Grants; Bioinformatics; Biological Markers; Biopsy Specimen; Cancer Center; Cancer Center Support Grant; Capital; Capital Financing; Categories; Communities; Data; Doctor of Philosophy; Educational workshop; Equipment; Faculty; Fees and Charges; Floor; Funding; Gel; Histology; Housing; Human; Human Resources; Image; Individual; Laboratories; Learning; Link; Lipids; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Medical Research; Microscope; Mission; NCI Center for Cancer Research; Pharmaceutical Preparations; Physiological; Postdoctoral Fellow; Process; Program Research Project Grants; Proteins; Proteomics; Proteomics Shared Resource; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resolution; Resource Sharing; Resources; Robot; Services; Site Visit; Software Tools; Structure; Techniques; Technology; Time; Tissues; Training; Training Programs; United States National Institutes of Health; Universities; Visit; base; cost; cost effective; design; digital; fluorescence imaging; graduate student; improved; instrument; instrumentation; laboratory facility; lectures; mass spectrometer; member; novel; programs; protein profiling; research study; small molecule; square foot; tool; whole animal imaging

The mission of the Biomolecular & Proteomics Shared Resource (BPSR) is to provide cost-effective, state-of-the-art instrumentation and analytical expertise to investigators in the Vanderbilt-lngram Cancer Center (VICC). The shared resource consists of three specialized laboratories dedicated to analysis of small molecules (i.e., drugs, drug metabolites and endogenous metabolites) In tissues and physiologic fluids; a comprehensive analytical proteomics facility for identification, characterization and quantitation of proteins; and a mass spectrometry imaging and histology-directed protein profiling facility. At the time of the last competitive renewal, these activities were separated into three independent shared resources, each receiving an "Outstanding" rating. Combining them into a single administrative structure has improved efficiency and service, while cutting costs. The facility currently houses 17 mass spectrometers. Other specialized capital equipment for imaging and proteomic studies are also available, such as high resolution matrix spotters, a cryomacrotome for whole animal imaging studies, a high resolution digital microscope for documenting histology data, a 2D gel fluorescence imaging and 2D gel processing robot. A bioinformatics support staff develops new software tools to support the shared resource and maintains the computing infrastructure. Justifications for including this shared resource in the CCSG proposal include (1) the extensive use of mass spectrometry services by the VICC research community, (2) the unique and comprehensive array of services offered to investigators, and (3) the cost-effective delivery of these services by a highly skilled professional staff. In FY 2008, 95 VICC/CCSG research groups used these services. The shared resource provides 16 categories of analytical services covering a wide range of analytical measurements from drug quantitation for PK/PD studies; identification, characterization and quantitation of cancer biomarkers; and imaging and profiling of proteins, lipids and drugs in human biopsy samples. The shared resource is supported by the CCSG, by research grants to individual investigators, and by significant institutional support from Vanderbilt.

生物分子和蛋白质组学共享资源（BPSR）的使命是提供具有成本效益的， 范德比尔特癌癌症研究人员的最先进的仪器和分析专业知识 中心（VICC）。共享资源由三个专门用于分析小型的专业实验室组成 组织和生理液中的分子（即药物，药物代谢产物和内源代谢产物）；一个 用于鉴定，表征和定量蛋白质的综合分析蛋白质组学设施； 以及质谱成像和组织学指导的蛋白质分析设施。最后一个 竞争性更新，将这些活动分为三个独立共享资源，每个资源 获得“未偿”评级。将它们组合成单个行政结构已改善 效率和服务，同时削减成本。该设施目前容纳17个质谱仪。其他 还提供用于成像和蛋白质组学研究的专门资本设备，例如高分辨率 矩阵示例，一种用于全动物成像研究的冷冻剖面，是一种高分辨率数字显微镜 记录组织学数据，2D凝胶荧光成像和2D凝胶处理机器人。生物信息学 支持人员开发了新的软件工具来支持共享资源并维护计算 基础设施。 CCSG建议中包含此共享资源的理由包括（1） VICC研究社区广泛使用质谱服务，（2）独特和 向调查人员提供的全面服务，以及（3）这些服务的具有成本效益的交付 由高技能的专业员工。在2008财年，有95个VICC/CCSG研究小组使用了这些服务。这 共享资源提供16种涵盖广泛分析的分析服务 来自PK/PD研究的药物定量测量；识别，表征和定量 癌症生物标志物；以及人类活检样品中蛋白质，脂质和药物的成像和分析。这 共享资源得到了CCSG的支持，研究赠款给个人调查员以及大量 范德比尔特的机构支持。