FACS Aria II Three Laser Special Order System

描述（由申请人提供）：内布拉斯加大学-林肯分校（UNL）流式细胞术核心机构申请资金，以将其FACS Vantage SE流式细胞仪/细胞分选仪（已过时）更换为FACS Aria II三激光特殊订单系统。这种新仪器将提供细胞分选和数据分析能力，超越现有仪器目前的四色限制。这一升级将提高核心的分选能力，并为需要进行四种以上荧光参数分析和/或提高荧光蛋白和绿色可激发染料灵敏度的研究人员提供额外的激光激发/发射参数。仪器用户：流式细胞术核心设施服务于整个UNL校园，并由内布拉斯加州病毒学中心（NCV）管理。它的用户群正在增加。它目前的主要用户群包括来自UNL几个部门的12名研究人员，他们具有不同的应用，包括生物科学，兽医和生物医学科学，生物化学，食品科学和技术以及生物系统工程。八个主要用户是NIH资助的研究人员;这些研究人员中至少有五个在仪器安装时将有NIH资助的研究项目，这些项目将直接受益于仪器。此外，已经确定了三个小用户，他们使用这种设备将产生初步数据，用于待定和拟议的研究赠款，几个NIH资助的培训赠款的学员将受益于使用仪器的研究经验。意义：流式细胞仪Aria将是UNL和内布拉斯加州林肯市唯一的细胞分选仪。这对促进许多研究人员的职业发展至关重要，特别是那些隶属于NCV和内布拉斯加州氧化还原生物学中心的研究人员，NIH生物医学研究卓越中心部分负责发展有前途的初级研究人员的职业生涯。将该设备添加到核心设施将为该设施的许多当前NIH资助的用户提供新的研究途径，并提高新的和年轻的研究人员成为NIH资助的研究赠款的主要研究人员的能力，并从其他机构获得研究资金。最后，新的细胞分选能力将取代核心设施中老化和过时的FACS Vantage，这将影响UNL的本科生和研究生以及博士后研究员的研究培训，并为该市其他教育机构的教师提供新的机会，利用最先进的科学仪器进行研究。 公共卫生相关性：购买一台FACS Aria II三激光特殊订单系统是必要的，以促进内布拉斯加大学林肯分校（UNL）众多NIH资助的研究人员的研究计划，推动UNL有前途的初级研究人员获得NIH资助，并改善UNL的本科生和研究生以及博士后研究员的研究培训。FACS Aria将取代现已过时的FACS Vantage SE流式细胞仪/细胞分选仪。鉴于这将是内布拉斯加州林肯唯一的细胞分类器，它还将为该市其他教育机构的教师提供新的机会，利用最先进的科学仪器进行研究。

DESCRIPTION (provided by applicant): The University of Nebraska-Lincoln (UNL) flow cytometry core facility requests funds to replace its FACS Vantage SE flow cytometer/cell sorter (which is obsolete) with a FACS Aria II three laser special order system. This new instrumentation will provide cell sorting and data analysis capabilities beyond the current four color limitation of the existing instrumentation. This upgrade will enhance the sorting capabilities of the core and provide additional laser excitation/emission parameters to investigators requiring more than four fluorescent parameter analyses and/or increased sensitivity for fluorescent proteins and green excitable dyes. Instrumentation Users: The flow cytometry core facility serves the entire UNL campus and is being managed by the Nebraska Center for Virology (NCV). Its user base is increasing. Its current major user group comprises 12 researchers from several UNL departments with different applications, including biological sciences, veterinary and biomedical sciences, biochemistry, food science and technology, and biological systems engineering. Eight of the major users are NIH-funded investigators; at least five of these investigators will have active NIH-funded research programs at the time the instrument is installed that will directly benefit from the instrumentation. In addition, three minor users have been identified whose access to this equipment will generate preliminary data for pending and proposed research grants, and trainees in several NIH-funded training grants will benefit from research experience using the instrumentation. Significance: The FACS Aria will be the only cell sorter at UNL and in the city of Lincoln, Neb. and it is essential to further the development of numerous investigators' careers, especially those affiliated with the NCV and the Nebraska Redox Biology Center, NIH Centers for Biomedical Research Excellence charged in part with developing the careers of promising junior investigators. Addition of this equipment to the core facility will provide new avenues for research to many of the current NIH-funded users of the facility, as well as enhance the ability of new and young investigators to become principal investigators on NIH-funded research grants and obtain research funding from other agencies. Finally, the new cell sorting capability will replace the aging and obsolete FACS Vantage in the core facility, which will impact research training for UNL's undergraduate and graduate students and postdoctoral fellows, and provide new opportunities for faculty at other educational institutions in the city to perform research utilizing the state-of-the-science instrumentation. PUBLIC HEALTH RELEVANCE: Purchase of a FACS Aria II three laser special order system is necessary to further the research programs of numerous NIH-funded investigators at the University of Nebraska-Lincoln (UNL), propel promising junior investigators at UNL to NIH funding, and improve research training of UNL's undergraduate and graduate students and postdoctoral fellows. The FACS Aria will replace a FACS Vantage SE flow cytometer/cell sorter, which is now obsolete. Given that this will be the only cell sorter in Lincoln, Neb., it will also provide new opportunities for faculty at other educational institutions in the city to perform research utilizing the state-of-the-science instrumentation.