FLOW CYTOMETRY CORE

流式细胞术核心

• 批准号: 7959392
• 负责人: Charles Arthur Kuszynski
• 金额: $ 7.97万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959392
• 关键词: Antibodies; Apoptosis; Binding; Biological Assay; Caspase; Cell Cycle; Cell Separation; Cells; Clone Cells; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Custom; DNA analysis; Data; Data Analyses; Data Collection; Education; Flow Cytometry; Flow Cytometry Shared Resource; Funding; Goals; Grant; Immunofluorescence Immunologic; Institution; Kinetics; Nebraska; Preparation; Proteins; Research; Research Personnel; Resource Sharing; Resources; Sampling; Services; Source; Specimen Handling; Surface; Techniques; Time Study; Transduction Gene; United States National Institutes of Health; annexin A5; cancer therapy; interest; protocol development; receptor expression; theories; virology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Flow Cytometry Shared Resource provides researcher access to flow cytometry and cell sorting. The goal of the Cell Analysis Shared Resource is to provide comprehensive data collection, data analysis, data interpretation and education in flow cytometry to all interested investigators. This includes proper sample preparation as well as education in theory and practice of flow cytometry. The Flow Cytometry Shared Resource provides basic direction on the set up and processing of specimens for flow analysis. These include: immunofluorescence (1-15 colors, 1-17 parameters); surface expression of standard CD markers; specialized expression of surface markers with custom antibodies; gene transduction/expression; fluorescent proteins; receptor expression; apoptosis; Annexin V binding; caspase activity; DNA analysis; cell cycle assays; apoptosis; cancer therapy; kinetic studies (time dependent assay); cell sorting; cell enrichment; cell purification; single cell cloning; new protocol development; and preliminary studies to bring new techniques into the repertoire of available services of the Flow Cytometry Shared Resource. The Flow Cytometry Shared Resource provides data analysis of flow cytometric data utilizing the most current versions of analysis software.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 流式细胞术共享资源为研究人员提供了流式细胞术和细胞分选的访问权限。细胞分析共享资源的目标是向所有感兴趣的研究人员提供流式细胞术方面的全面数据收集、数据分析、数据解释和教育。这包括适当的样品制备以及流式细胞术理论和实践的教育。流式细胞术共享资源提供了流式分析样本的设置和处理的基本指导。其中包括：免疫荧光（1-15 种颜色，1-17 个参数）；标准CD标记的表面表达；使用定制抗体专门表达表面标记；基因转导/表达；荧光蛋白；受体表达；细胞凋亡；膜联蛋白 V 结合；胱天蛋白酶活性； DNA分析；细胞周期测定；细胞凋亡；癌症治疗；动力学研究（时间依赖性测定）；细胞分选；细胞富集；细胞纯化；单细胞克隆；新协议开发；以及将新技术纳入流式细胞术共享资源可用服务的初步研究。流式细胞术共享资源利用最新版本的分析软件提供流式细胞术数据的数据分析。