FLOW CYTOMETRY CORE

流式细胞术核心

• 批准号: 7381209
• 负责人: Charles Arthur Kuszynski
• 金额: $ 8.46万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381209
• 关键词: FLOW; CYTOMETRY; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Flow Cytometry Core Facility is a newly organized core that is now fully functioning and provides NCV members and associates with technical and functional support for flow cytometry. There are two locations for the instrumentation controlled by the core, the BL3 facility in the Beadle Center and the flow cytometry lab in the Veterinary Basic Science building. A sister core laboratory is located in the Durham Center for Research on the University of Nebraska Medical Center Campus. The facility now consists of a FACSCalibur, four color bench top analysis instrument, a FACScan, three color bench top analysis instrument and a FACS Vantage 6 color cell sorting instrument, all from Becton Dickinson Biosciences. These instruments provide a wide range of applications for NCV investigators, including immunofluorescence, bead capture assays, DNA profiles and cell sorting. The data are collected and stored as list mode files on site in the core. All data is analyzed and returned to the investigators for their approval. In addition, in depth discussions regarding new protocol development are an ongoing process. During this year, consultations have taken place leading to new grant submissions and publication submissions. Currently there are two echnicians, one at each location to provide support and acquire data.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。流式细胞术核心设施是一个新组建的核心设施，现已全面运作，为 NCV 成员和同事提供流式细胞术的技术和功能支持。由核心控制的仪器有两个位置：Beadle 中心的 BL3 设施和兽医基础科学大楼的流式细胞术实验室。姐妹核心实验室位于内布拉斯加大学医学中心校区的达勒姆研究中心。该设施现在包括一台 FACSCalibur（四色台式分析仪）、一台 FACScan（三色台式分析仪）和一台 FACS Vantage 6 色细胞分选仪（全部来自 Becton Dickinson Biosciences）。这些仪器为 NCV 研究人员提供了广泛的应用，包括免疫荧光、珠子捕获分析、DNA 谱和细胞分选。数据被收集并作为列表模式文件存储在核心现场。所有数据均经过分析并返回给研究人员以供批准。此外，有关新协议开发的深入讨论仍在进行中。今年，我们进行了磋商，提交了新的赠款和出版物。目前有两名技术人员，每个地点一名，负责提供支持和获取数据。