CORE--Cell Analysis

核心--细胞分析

• 批准号: 7484291
• 负责人: Charles Arthur Kuszynski
• 金额: $ 5.14万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7484291
• 关键词: Antibodies; Apoptosis; Binding; Biochemistry; Biological Assay; Caspase; Cell Cycle; Cell Separation; Cells; Clone Cells; Color; Computer software; Custom; DNA analysis; Data; Data Analyses; Data Collection; Education; Flow Cytometry; Goals; Green Fluorescent Proteins; Immunofluorescence Immunologic; Kinetics; Library Protocol; Molecular Biology; Preparation; Proteins; Publications; Research Personnel; Resource Sharing; Sampling; Services; Specimen Handling; Speed; Standards of Weights and Measures; Students; Surface; Techniques; Time Study; Tissues; Training; Transduction Gene; annexin A5; cancer therapy; cell preparation; fluorophore; interest; lectures; protocol development; receptor expression; theories

DESCRIPTION (provided by applicant): The Cell Analysis Shared Resource provides researcher access to flow cytometry and cell sorting. The goal of the Cell Analysis Shared Resource is to provide comprehensive data collection, data analysis, data interpretation and education in flow cytometry to all interested investigators. This includes proper sample preparation as well as education in theory and practice of flow cytometry. The Cell Analysis Shared Resource provides basic direction on the set up and processing of specimens for flow analysis. These include: immunofluorescence (1-4 colors, 1-7 parameters); surface expression of standard CD markers; specialized expression of surface markers with custom antibodies; gene transduction/expression; green fluorescent protein; receptor expression; apoptosis; Annexin V binding; caspase activity; DNA analysis; cell cycle assays; apoptosis; cancer therapy; kinetic studies (time dependent assay); cell sorting; cell enrichment; cell purification; single cell cloning; new protocol development; and preliminary studies to bring new techniques into the repertoire of available services of the Cell Analysis Shared Resource. The Cell Analysis Shared Resource provides data analysis of flow cytometric data utilizing the most current versions of analysis software.

描述（由申请人提供）：细胞分析共享资源为研究人员提供 获得流式细胞术和细胞分选。细胞分析共享资源的目标是向所有感兴趣的研究人员提供流式细胞术方面的全面数据收集、数据分析、数据解释和教育。这包括适当的样品制备以及流式细胞术理论和实践的教育。细胞分析共享资源提供了流式分析样本的设置和处理的基本指导。其中包括：免疫荧光（1-4 种颜色，1-7 个参数）；标准CD标记的表面表达；使用定制抗体专门表达表面标记；基因转导/表达；绿色荧光蛋白；受体表达； 细胞凋亡；膜联蛋白 V 结合；胱天蛋白酶活性； DNA分析；细胞周期测定；细胞凋亡；癌症治疗；动力学研究（时间依赖性测定）；细胞分选；细胞富集；细胞纯化；单细胞克隆；新协议开发；以及将新技术纳入细胞分析共享资源可用服务的初步研究。细胞分析共享资源利用最新版本的分析软件提供流式细胞术数据的数据分析。