Transmission Electron Microscope in the Academic Health Center

描述(由申请人提供)：这是明尼苏达大学学术卫生中心将使用的新的120KV基本透射式电子显微镜的共享仪器资助申请。这项请求的主要目的是更换陈旧的电子显微镜，巩固这些仪器的使用，并振兴该大学、明尼苏达州和该地区的生物电子显微镜科学。获得这一基本仪器对于振兴电子显微镜作为该大学结构生物学的重要组成部分以及保持一流的研究企业至关重要。六名拟使用电子显微镜的人是牙科学院的研究人员，四人是医学院的教职员工。五名使用者是分子病毒学研究所的成员。拟议的项目(由研究人员进行)将利用电子显微镜来1)确定枯草杆菌噬菌体x29的组装结构和分子机制、动物病毒组装的模型和确定抗病毒药物靶标的方法；2)确定糖尿病和其他疾病患者肾脏的结构和功能之间的关系；3)研究具有重要感觉和运动功能的真核纤毛和鞭毛的结构和组装；4)研究钙保护素在角质形成细胞中的定位、HIV-1的跨粘膜转移、角质形成细胞的微生物入侵和粘附素在生物膜形成中的表达；5)确定HIV-1变异和颗粒释放的机制；6)研究蛋白酶体的调节颗粒；7)确定粘膜T细胞对共生微生物的跟踪；8)研究甲型病毒膜融合机制。

DESCRIPTION (provided by applicant): This is a Shared Instrumentation Grant Application for a new 120kV basic transmission electron microscope (TEM) to be used at the Academic Health Center of the University of Minnesota. The primary purposes of this request are to replace obsolete TEMs, consolidate the use of these instruments, and reinvigorate the science of biological electron microscopy in the University, the State of Minnesota, and the region. The acquisition of this basic instrument is critical in revitalizing electron microscopy as an essential component of structural biology at the university and in maintaining a first-class research enterprise. Six proposed users of the TEM are investigators in the School of Dentistry, and four are faculty members in the School of Medicine. Five users are members of the Institute for Molecular Virology. The proposed projects (by investigator) will use the TEM to 1) determine the structure and molecular mechanisms of assembly of bacteriophage X29 of Bacillus subtilis, a model for assembly of animal viruses and a means to identify targets for antiviral agents; 2) determine the relationship between structure and function in kidneys from patients with diabetes and other diseases; 3) study the structure and assembly of Eukaryotic cilia and flagella that serve vital sensory and motile functions; 4) study localization of calprotectin in keratinocytes, transmucosal transfer of HIV-1, microbial invasion of keratinocytes, and adhesin expression in biofilm formation; 5) determine the mechanisms of HIV-1 variation and particle release; 6) study the regulatory particle of the proteasome; and 7) determine the tracking of mucosal T cells to commensal microbes; 8) study the Alphavirus membrane fusion mechanism.