High Capacity UltraFISH for Single-cell Spatial Transcriptomics

SBIR摘要 单细胞生物学是一门多学科的研究，旨在研究 单个细胞中分子物种之间的相互作用以及大量细胞群体如何工作 作为一个复杂的系统。识别基因表达的差异和空间差异 在单细胞水平上组织组织对于破译组织异质性是必不可少的， 器官发育，并深刻了解生物系统，并最终受益 精准、个性化医疗。目前，单细胞RNA测序被广泛应用于 分析单个细胞的基因表达模式。然而，单细胞RNA测序具有 重大局限性，包括缺乏空间信息、RNA检测效率低和 细胞捕获效率。现有的多路复用原位检测技术，如MerFish和 SeqFISH可以克服这些限制，但有很长的周转时间。UltraFISH，由 彩虹诊断，显著缩短了一小块RNA面板的周转时间 现场检测。大容量UltraFISH是为了进一步提升网络的多路传输能力 UltraFISH使一个大的RNA面板如整个人类转录组 可以在几小时内高效、快速地分析组织区域。随着成功的结果 这项提议，高容量UltraFISH将给单细胞分析领域带来革命性的变化，导致 在不久的将来，我们对生物学和疾病机制的理解将取得突破。

SBIR Abstract Single cell biology is a multi-disciplinary study to investigate how large amount of molecular species interact with each other in single cells and how large population of cells work together as a complex system. Identifying the difference of gene expression and the spatial organization of tissues at the single-cell level is essential to decipher tissue heterogeneity, organ development, and understand the biological systems profoundly, and ultimately benefit precision and personalized medicine. Currently, single cell RNA sequencing is widely used to analyze the gene expression patterns of single cells. However, single cell RNA sequencing has significant limitations, including lack of spatial information, low RNA detection efficiency and low cell capture efficiency. Existing multiplexed in-situ detection technologies like MERFISH and seqFISH can overcome these limitations but has long turnaround time. UltraFISH, developed by Rainbow Diagnostics, significantly improves the turnaround time for a small panel of RNA detection in-situ. High Capacity UltraFISH is to further advance the multiplex capacity of UltraFISH so that a large RNA panel such as the whole human transcriptome from a large region of tissue can be efficiently and quickly profiled in hours. With the successful outcome of this proposal, High Capacity UltraFISH will revolutionize the single cell analysis field, leading to breakthroughs in our understanding of biological and disease mechanisms in the near future.