Cluster Ion Source for ESCA Instrument

ESCA 仪器的簇离子源

基本信息

  • 批准号:
    7791458
  • 负责人:
  • 金额:
    $ 11.5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-01-07 至 2011-01-06
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Many important cell and tissue functions depend on the surface presentation of molecules, so it is critical to determine the chemical state (composition, molecular structure, and orientation) as well as the distribution of biological moieties present on a surface and in the near surface region of biomedical devices. Thus, it is essential to develop surface analysis techniques capable of providing quantitative and detailed surface chemical state information at high spatial resolutions, both for 2-D imaging of surfaces and 3-D imaging of the near surface region. Until recently it was not possible with electron spectroscopy for chemical analysis (ESCA, also known as x-ray photoelectron spectroscopy or XPS) to obtain 3-D images of biological materials by sputter depth profiling. This is because the commercial ion sources available on ESCA instruments produced monoatomic species such as Ar+ that resulted in significant sample degradation and loss of molecular information during sputtering. A few years ago commercial cluster ion sources such C60+ became available on time-of-flight secondary ion mass spectrometry (ToF-SIMS) instruments. These sources were capable of molecular depth profiling of biological materials and opened the possibility of 3-D imaging with ToF-SIMS. Building on the success these cluster ion beam sources have experienced in the ToF-SIMS community, they are now being developed for commercial ESCA instruments. In the Fall of 2008 the manufacturer of our imaging ESCA instrument at the University of Washington (Kratos Analytical) introduced a cluster ion beam source for their AxisUltra DLD instrument, which now makes ESCA molecular depth profiling and 3-D imaging of biological materials possible. To capitalize on these new capabilities and the benefits they will provide to NIH-funded research projects, we are requesting $115,000 to upgrade our existing Kratos Analytical AxisUltra DLD ESCA instrument with the new cluster ion beam source. ESCA and ToF-SIMS are complementary surface analysis techniques. ESCA provides quantitative information about elemental and chemical species composition, while ToF-SIMS provides more detailed molecular structure information. Thus, combined 3-D imaging with both ESCA and ToF-SIMS provide detailed quantitative and molecular structure information about a sample. Research projects that will benefit from the new 3-D ESCA imaging capability include analysis of medical implants (e.g., drug-loaded/coated stents), biofilms, cell sheets, tissue sections, tissue engineering scaffolds, microarray devices (DNA, antibody, carbohydrate, etc.), biosensors, etc. HEALTH RELEVANCE: Knowledge about biomedical implant surfaces and an understanding of how these surfaces interact and direct biological processes when such a device is placed in the body is needed to permit the rational design of new and improved biomedical implants. The proposed cluster ion beam source will provide new information about the surface properties of biomedical devices that is needed to achieve this objective.
描述(由申请人提供):许多重要的细胞和组织功能依赖于分子的表面呈现,因此确定生物医学设备表面和近表面区域存在的生物部分的化学状态(组成,分子结构和取向)以及分布至关重要。因此,开发能够在高空间分辨率下提供定量和详细的表面化学状态信息的表面分析技术至关重要,无论是表面的二维成像还是近表面区域的三维成像。直到最近,化学分析电子能谱(ESCA,也称为x射线光电子能谱或XPS)还不可能通过溅射深度剖面获得生物材料的三维图像。这是因为ESCA仪器上可用的商业离子源产生单原子物种,如Ar+,导致样品在溅射过程中严重降解和分子信息丢失。几年前,像C60+这样的商业集群离子源在飞行时间二次离子质谱(ToF-SIMS)仪器上可用。这些资源能够对生物材料进行分子深度分析,并开启了使用ToF-SIMS进行三维成像的可能性。基于这些簇离子束源在ToF-SIMS社区中取得的成功,它们现在正在开发用于商用ESCA仪器。2008年秋天,我们在华盛顿大学(Kratos Analytical)的成像ESCA仪器的制造商为他们的AxisUltra DLD仪器引入了一个簇离子束源,现在使ESCA分子深度分析和生物材料的3-D成像成为可能。为了利用这些新功能以及它们将为nih资助的研究项目提供的好处,我们申请115,000美元,用新的集群离子束源升级我们现有的Kratos Analytical AxisUltra DLD ESCA仪器。ESCA和ToF-SIMS是互补的表面分析技术。ESCA提供元素和化学物种组成的定量信息,而ToF-SIMS提供更详细的分子结构信息。因此,结合ESCA和ToF-SIMS的三维成像提供了样品的详细定量和分子结构信息。将受益于新的3-D ESCA成像能力的研究项目包括分析医疗植入物(例如,载药/涂层支架)、生物膜、细胞片、组织切片、组织工程支架、微阵列设备(DNA、抗体、碳水化合物等)、生物传感器等。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

DAVID G CASTNER其他文献

DAVID G CASTNER的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('DAVID G CASTNER', 18)}}的其他基金

MS Chemical Imager Instrument
MS化学成像仪
  • 批准号:
    8730331
  • 财政年份:
    2014
  • 资助金额:
    $ 11.5万
  • 项目类别:
Cluster Ion Sources for ToF-SIMS
用于 ToF-SIMS 的簇离子源
  • 批准号:
    8246268
  • 财政年份:
    2012
  • 资助金额:
    $ 11.5万
  • 项目类别:
ToF-SIMS Instrument
ToF-SIMS仪器
  • 批准号:
    7040267
  • 财政年份:
    2006
  • 资助金额:
    $ 11.5万
  • 项目类别:
Nanoscale Investigation of Protein-Surface Interactions
蛋白质-表面相互作用的纳米级研究
  • 批准号:
    7578839
  • 财政年份:
    2006
  • 资助金额:
    $ 11.5万
  • 项目类别:
Nanoscale Investigation of Protein-Surface Interactions
蛋白质-表面相互作用的纳米级研究
  • 批准号:
    7176171
  • 财政年份:
    2006
  • 资助金额:
    $ 11.5万
  • 项目类别:
TOF-SIMS INSTRUMENT
TOF-SIMS仪器
  • 批准号:
    7335089
  • 财政年份:
    2006
  • 资助金额:
    $ 11.5万
  • 项目类别:
Nanoscale Investigation of Protein-Surface Interactions
蛋白质-表面相互作用的纳米级研究
  • 批准号:
    7038736
  • 财政年份:
    2006
  • 资助金额:
    $ 11.5万
  • 项目类别:
Nanoscale Investigation of Protein-Surface Interactions
蛋白质-表面相互作用的纳米级研究
  • 批准号:
    7350156
  • 财政年份:
    2006
  • 资助金额:
    $ 11.5万
  • 项目类别:
Clinical Research Experience for Engineers (CREE)(RMI)
工程师临床研究经验 (CREE)(RMI)
  • 批准号:
    7483044
  • 财政年份:
    2004
  • 资助金额:
    $ 11.5万
  • 项目类别:
Clinical Research Experience for Engineers (CREE)(RMI)
工程师临床研究经验 (CREE)(RMI)
  • 批准号:
    6951457
  • 财政年份:
    2004
  • 资助金额:
    $ 11.5万
  • 项目类别:

相似海外基金

University of Aberdeen and Vertebrate Antibodies Limited KTP 23_24 R1
阿伯丁大学和脊椎动物抗体有限公司 KTP 23_24 R1
  • 批准号:
    10073243
  • 财政年份:
    2024
  • 资助金额:
    $ 11.5万
  • 项目类别:
    Knowledge Transfer Partnership
Role of Natural Antibodies and B1 cells in Fibroproliferative Lung Disease
天然抗体和 B1 细胞在纤维增生性肺病中的作用
  • 批准号:
    10752129
  • 财政年份:
    2024
  • 资助金额:
    $ 11.5万
  • 项目类别:
CAREER: Next-generation protease inhibitor discovery with chemically diversified antibodies
职业:利用化学多样化的抗体发现下一代蛋白酶抑制剂
  • 批准号:
    2339201
  • 财政年份:
    2024
  • 资助金额:
    $ 11.5万
  • 项目类别:
    Continuing Grant
Isolation and characterisation of monoclonal antibodies for the treatment or prevention of antibiotic resistant Acinetobacter baumannii infections
用于治疗或预防抗生素耐药鲍曼不动杆菌感染的单克隆抗体的分离和表征
  • 批准号:
    MR/Y008693/1
  • 财政年份:
    2024
  • 资助金额:
    $ 11.5万
  • 项目类别:
    Research Grant
Developing first-in-class aggregation-specific antibodies for a severe genetic neurological disease
开发针对严重遗传神经系统疾病的一流聚集特异性抗体
  • 批准号:
    10076445
  • 财政年份:
    2023
  • 资助金额:
    $ 11.5万
  • 项目类别:
    Grant for R&D
Discovery of novel nodal antibodies in the central nervous system demyelinating diseases and elucidation of the mechanisms through an optic nerve demyelination model
发现中枢神经系统脱髓鞘疾病中的新型节点抗体并通过视神经脱髓鞘模型阐明其机制
  • 批准号:
    23K14783
  • 财政年份:
    2023
  • 资助金额:
    $ 11.5万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Elucidation of the mechanisms controlling the physicochemical properties and functions of supercharged antibodies and development of their applications
阐明控制超电荷抗体的理化性质和功能的机制及其应用开发
  • 批准号:
    23KJ0394
  • 财政年份:
    2023
  • 资助金额:
    $ 11.5万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Role of antibodies in hepatitis E virus infection
抗体在戊型肝炎病毒感染中的作用
  • 批准号:
    10639161
  • 财政年份:
    2023
  • 资助金额:
    $ 11.5万
  • 项目类别:
Defining the protective or pathologic role of antibodies in Post-Ebola Syndrome
定义抗体在埃博拉后综合症中的保护或病理作用
  • 批准号:
    10752441
  • 财政年份:
    2023
  • 资助金额:
    $ 11.5万
  • 项目类别:
Human CMV monoclonal antibodies as therapeutics to inhibit virus infection and dissemination
人 CMV 单克隆抗体作为抑制病毒感染和传播的治疗药物
  • 批准号:
    10867639
  • 财政年份:
    2023
  • 资助金额:
    $ 11.5万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了