MS Chemical Imager Instrument

MS化学成像仪

• 批准号: 8730331
• 负责人: DAVID G CASTNER
• 金额: $ 174.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8730331
• 关键词: Antibodies; Biocompatible Materials; Biological; Biomedical Research; Biosensor; Carbohydrates; Cell Culture Techniques; Cell physiology; Cells; Chemicals; Communities; Complex; DNA; Development; Devices; Freeze Fracturing; Image; Ions; Microbial Biofilms; Molecular; Molecular Structure; Pharmaceutical Preparations; Physiologic pulse; Process; Research Personnel; Research Project Grants; Resolution; Sampling; Source; Spatial Distribution; Spectrometry, Mass, Secondary Ion; Stents; Structure; Surface; Techniques; Three-Dimensional Imaging; Time; Tissue Engineering; Tissues; Width; design; instrument; instrumentation; interest; ion source; mass analyzer; medical implant; research study; scaffold

DESCRIPTION (provided by applicant): Many important cell functions depend on the surface presentation of molecules, so it is critical to determine the chemical state (composition, molecular structure, and orientation) and spatial distribution of biological moieties present in th near surface region of biomedical devices, as well as in the cells and tissues themselves. This requires development of surface analysis techniques capable of providing detailed surface chemical state information at high spatial resolutions both for 2-D imaging of surfaces and 3-D imaging of cells and tissues. A few years ago commercial cluster ion sources became available on time-of-flight secondary ion mass spectrometry (ToF-SIMS) instruments. These sources were capable of molecular depth profiling biological materials and opened the possibility of 3-D imaging with ToF-SIMS. This has resulted in a revolutionary change in the ToF-SIMS community and significantly increased interest of biomedical researchers in ToF-SIMS. The newly developed J105 Chemical Imager, a ToF-SIMS instrument with new capabilities, presents yet another significant advance in ToF-SIMS instrumentation for biomedical research. Its design represents several important advances over current commercial ToF-SIMS instruments: the decoupling of mass analysis from the primary ion beam sputtering process, a significantly larger depth of field for extraction of secondary ions, and the ability to do MS/MS experiments. In a traditional commercial ToF-SIMS instrument the spectral mass resolution is defined by the pulse width of the primary ion beam. In the J105 Chemical Imager, the mass analyzer defines the spectral mass resolution. This means direct current or long pulsed primary ion beams can be used, resulting in a 103 increase in count rates and the ability to acquire images with both high mass and spatial resolution. The large extraction depth of field means high-quality, SEM-like images can be obtained from rough and topologically complex samples such as stents and porous scaffolds. MS/MS experiments provide more detailed information about the composition and structure of ions. Now for the first time in a commercial ToF-SIMS instrument, the enhanced benefits of MS/MS analysis are available. An additional advantage of the J105 Chemical Imager is the MousetrapTM freeze fracture sample holder for analysis of hydrated biological materials. Research projects that will benefit from the new capabilities of the new J105 Chemical Imager ToF-SIMS instrument include analysis of medical implants (e.g., drug-loaded/coated stents), biofilms, cells, tissue sections, cell culture devices, tissue engineering scaffolds, microarray devices (DNA, antibody, carbohydrate, etc.), biosensors, etc.

描述（由申请人提供）：许多重要的细胞功能取决于分子的表面表现，因此确定化学状态（组成，分子结构和方向）以及在生物医学设备的近表面区域以及细胞和组织本身的生物学部分的空间分布至关重要。这需要开发能够在高空间分辨率下提供详细的表面化学状态信息的表面分析技术，以供表面2-D成像和细胞和组织的3-D成像。几年前，商业集群离子源可在飞行时间二级离子质谱（TOF-SIMS）仪器上获得。这些来源能够分子深度分析生物材料，并用TOF-SIMS打开了3D成像的可能性。这导致了TOF-SIMS社区的革命性变化，并显着增加了生物医学研究人员对TOF-SIM的兴趣。新开发的J105化学成像仪是一种具有新功能的TOF-SIMS仪器，在TOF-SIMS仪器中为生物医学研究提供了另一个重大进步。它的设计代表了当前商业TOF-SIMS仪器的几个重要进展：与原代离子束溅射过程中质量分析的解耦，二次离子提取的景深明显更大，并且具有进行MS/MS实验的能力。在传统的商业TOF-SIMS仪器中，光谱质量分辨率由原代离子束的脉冲宽度定义。在J105化学成像仪中，质量分析仪定义了光谱质量分辨率。这意味着可以使用直流电流或长脉冲的原代离子束，从而导致计数率提高103，并且具有高质量和空间分辨率的图像的能力。较大的景深意味着可以从粗糙和拓扑复杂的样品（例如支架和多孔脚手架）中获得高质量的SEM样图像。 MS/MS实验提供了有关离子组成和结构的更详细信息。现在，在商业TOF-SIMS仪器中首次获得了MS/MS分析的增强好处。 J105化学成像仪的另一个优点是Mousetraptm Freeze断裂样品支架用于分析水合生物材料。研究项目将受益于新的J105化学成像仪TOF-SIMS仪器的新功能，包括分析医疗植入物（例如药物负载/涂层的支架），生物膜，细胞，组织切片，细胞培养设备，组织工程型号，组织工程型号，微阵列，微阵列设备（DNA，抗体，抗体，抗体，抗体，抗体型），生物体，等等，Ack，Ack，ACK，ACK，ACK，ACK，ACK，ACK，ACK，ACK，ACK，ACK，ACK，ACK，ACK，ACK