MS Chemical Imager Instrument

MS化学成像仪

• 批准号: 8730331
• 负责人: DAVID G CASTNER
• 金额: $ 174.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8730331
• 关键词: Antibodies; Biocompatible Materials; Biological; Biomedical Research; Biosensor; Carbohydrates; Cell Culture Techniques; Cell physiology; Cells; Chemicals; Communities; Complex; DNA; Development; Devices; Freeze Fracturing; Image; Ions; Microbial Biofilms; Molecular; Molecular Structure; Pharmaceutical Preparations; Physiologic pulse; Process; Research Personnel; Research Project Grants; Resolution; Sampling; Source; Spatial Distribution; Spectrometry, Mass, Secondary Ion; Stents; Structure; Surface; Techniques; Three-Dimensional Imaging; Time; Tissue Engineering; Tissues; Width; design; instrument; instrumentation; interest; ion source; mass analyzer; medical implant; research study; scaffold

DESCRIPTION (provided by applicant): Many important cell functions depend on the surface presentation of molecules, so it is critical to determine the chemical state (composition, molecular structure, and orientation) and spatial distribution of biological moieties present in th near surface region of biomedical devices, as well as in the cells and tissues themselves. This requires development of surface analysis techniques capable of providing detailed surface chemical state information at high spatial resolutions both for 2-D imaging of surfaces and 3-D imaging of cells and tissues. A few years ago commercial cluster ion sources became available on time-of-flight secondary ion mass spectrometry (ToF-SIMS) instruments. These sources were capable of molecular depth profiling biological materials and opened the possibility of 3-D imaging with ToF-SIMS. This has resulted in a revolutionary change in the ToF-SIMS community and significantly increased interest of biomedical researchers in ToF-SIMS. The newly developed J105 Chemical Imager, a ToF-SIMS instrument with new capabilities, presents yet another significant advance in ToF-SIMS instrumentation for biomedical research. Its design represents several important advances over current commercial ToF-SIMS instruments: the decoupling of mass analysis from the primary ion beam sputtering process, a significantly larger depth of field for extraction of secondary ions, and the ability to do MS/MS experiments. In a traditional commercial ToF-SIMS instrument the spectral mass resolution is defined by the pulse width of the primary ion beam. In the J105 Chemical Imager, the mass analyzer defines the spectral mass resolution. This means direct current or long pulsed primary ion beams can be used, resulting in a 103 increase in count rates and the ability to acquire images with both high mass and spatial resolution. The large extraction depth of field means high-quality, SEM-like images can be obtained from rough and topologically complex samples such as stents and porous scaffolds. MS/MS experiments provide more detailed information about the composition and structure of ions. Now for the first time in a commercial ToF-SIMS instrument, the enhanced benefits of MS/MS analysis are available. An additional advantage of the J105 Chemical Imager is the MousetrapTM freeze fracture sample holder for analysis of hydrated biological materials. Research projects that will benefit from the new capabilities of the new J105 Chemical Imager ToF-SIMS instrument include analysis of medical implants (e.g., drug-loaded/coated stents), biofilms, cells, tissue sections, cell culture devices, tissue engineering scaffolds, microarray devices (DNA, antibody, carbohydrate, etc.), biosensors, etc.

描述（由申请人提供）：许多重要的细胞功能取决于分子的表面呈现，因此确定生物医学装置近表面区域以及细胞和组织本身中存在的生物部分的化学状态（组成、分子结构和方向）和空间分布至关重要。这需要开发能够以高空间分辨率提供详细的表面化学状态信息的表面分析技术，用于表面的 2D 成像和细胞和组织的 3D 成像。几年前，商用簇离子源出现在飞行时间二次离子质谱 (ToF-SIMS) 仪器上。这些来源能够对生物材料进行分子深度分析，并开启了利用 ToF-SIMS 进行 3D 成像的可能性。这导致了 ToF-SIMS 界的革命性变化，并显着增加了生物医学研究人员对 ToF-SIMS 的兴趣。新开发的 J105 化学成像仪是一款具有新功能的 ToF-SIMS 仪器，代表了生物医学研究 ToF-SIMS 仪器的又一重大进步。其设计代表了当前商用 ToF-SIMS 仪器的几个重要进步：质量分析与一次离子束溅射过程的分离、用于提取二次离子的显着更大的景深以及进行 MS/MS 实验的能力。在传统的商用 ToF-SIMS 仪器中，谱质量分辨率由一次离子束的脉冲宽度定义。在 J105 化学成像仪中，质量分析仪定义了光谱质量分辨率。这意味着可以使用直流或长脉冲初级离子束，从而使计数率提高 103 倍，并能够获取具有高质量和空间分辨率的图像。大提取景深意味着可以从支架和多孔支架等粗糙且拓扑复杂的样品中获得高质量的类似 SEM 的图像。 MS/MS 实验提供有关离子组成和结构的更详细信息。现在，商用 ToF-SIMS 仪器首次提供了 MS/MS 分析的增强优势。 J105 化学成像仪的另一个优势是用于分析水合生物材料的 MousetrapTM 冷冻断裂样品架。受益于新型 J105 化学成像仪 ToF-SIMS 仪器新功能的研究项目包括医疗植入物（例如载药/涂层支架）、生物膜、细胞、组织切片、细胞培养装置、组织工程支架、微阵列装置（DNA、抗体、碳水化合物等）、生物传感器等的分析。