Negative Regulation of Human Osteoclastogenesis

人类破骨细胞生成的负调控

基本信息

  • 批准号:
    8089430
  • 负责人:
  • 金额:
    $ 42.01万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-09-08 至 2012-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Periodontitis is a chronic infectious/inflammatory disease in which bacteria present in oral biofilm/dental plaque drive an inflammatory state leading to dysregulated remodeling and destruction of periodontal soft tissues and alveolar bone. This application will focus on mechanisms of inhibition of the generation of osteoclasts during inflammatory bone resorption. Cell activation and inflammatory mediator production in response to pathogenic oral bacteria is mediated by `pattern recognition receptors' that recognize conserved microbial molecules. Among the most important pattern recognition receptors are Toll like receptors 2 (TLR2) and TLR4 that have been implicated in driving inflammatory bone resorption in periodontitis. At the same time as they activate inflammation, TLRs induce potent homeostatic mechanisms to limit the intensity of inflammation and thus limit associated tissue damage. The extent of inflammation and the rate of associated bone loss in periodontitis are determined by the balance between inflammatory factors and the relative potency of feedback and homeostatic mechanisms that oppose inflammation and bone resorption, and promote healing. Thus, disease progression is evidence of relatively ineffective feedback inhibition that is not able to restrain inflammation and bone resorption, in contrast to effective homeostatic regulation that occurs during physiological resolution of inflammation or during quiescent phases of disease. Our overarching hypothesis is that augmenting physiological homeostatic mechanisms represents an effective approach to limiting bone resorption associated with inflammation. Therefore, we have initiated studies to understand molecular mechanisms that restrain osteoclast formation in an inflammatory setting such as periodontal disease. We have found that IL-10, a prototypic negative regulator that is induced by TLRs and widely expressed in periodontitis, suppresses osteoclastogenesis by inhibiting signaling by RANK and TREM-2, key receptors important for osteoclastogenesis. In addition, we found that TLRs directly suppress the formation of osteoclast precursors by inhibiting RANK and TREM-2 expression during development of osteoclast precursors. Interestingly, our results revealed qualitative and quantitative differences between the regulation of human and murine osteoclastogenesis, including differential regulation of TREM-2, that may help explain species differences in regulation of bone remodeling. In this application, we will investigate inhibitory/homeostatic pathways and molecular mechanisms by which IL-10 and TLRs inhibit human osteoclastogenesis. We will use human systems that are directly relevant for periodontitis pathogenesis and may reveal mechanisms that are not readily apparent in murine systems. We anticipate that our studies will yield insights into homeostatic regulation that can be exploited for therapeutic interventions to suppress alveolar bone resorption associated with periodontitis. Project Narrative: Periodontitis is a dental disease in which oral bacteria cause inflammation that destroys teeth. In this application we will investigate mechanisms that inhibit osteoclasts, the cells that destroy tooth bone in periodontitis. We anticipate that our studies will yield insights that can be exploited for therapeutic interventions to suppress the bone and tooth destruction associated with periodontitis.
描述(申请人提供):牙周炎是一种慢性感染性/炎症性疾病,口腔生物膜/牙菌斑中的细菌会导致炎症状态,导致牙周软组织和牙槽骨的失调重塑和破坏。这一应用将集中在炎症性骨吸收过程中抑制破骨细胞生成的机制。致病口腔细菌对细胞的激活和炎症介质的产生是由识别保守微生物分子的“模式识别受体”介导的。其中最重要的模式识别受体是Toll样受体2(TLR2)和TLR4,它们与牙周炎的炎症性骨吸收有关。在激活炎症的同时,TLRs诱导强大的动态平衡机制来限制炎症的强度,从而限制相关的组织损伤。牙周炎的炎症程度和相关的骨丢失率取决于炎症因子之间的平衡,以及对抗炎症和骨吸收并促进愈合的反馈和动态平衡机制的相对效力。因此,疾病进展是相对无效的反馈抑制的证据,不能抑制炎症和骨吸收,而有效的体内平衡调节发生在炎症的生理消退或疾病的静止期。我们的主要假设是,增强生理内平衡机制是限制与炎症相关的骨吸收的有效方法。因此,我们已经开始研究,以了解在炎症环境中抑制破骨细胞形成的分子机制,如牙周病。我们发现,IL-10是一种典型的负性调节因子,由TLRs诱导,并在牙周炎中广泛表达,它通过抑制RANK和TREM-2的信号转导来抑制破骨细胞的形成,RANK和TREM-2是破骨细胞发生的关键受体。此外,我们发现在破骨细胞前体的发育过程中,TLRs通过抑制RANK和TREM-2的表达直接抑制破骨细胞前体的形成。有趣的是,我们的结果揭示了人类和小鼠破骨细胞生成调控的质和量上的差异,包括对TREM-2的不同调控,这可能有助于解释骨重建调控的物种差异。在这项应用中,我们将研究IL-10和TLRs抑制人破骨细胞生成的抑制/内稳态途径和分子机制。我们将使用与牙周炎发病机制直接相关的人类系统,并可能揭示在小鼠系统中不太明显的机制。我们预计,我们的研究将对动态平衡调节产生洞察力,可用于治疗干预,以抑制与牙周炎相关的牙槽骨吸收。项目简介:牙周炎是一种口腔疾病,口腔细菌会引起炎症,破坏牙齿。在这项应用中,我们将研究抑制破骨细胞的机制,破骨细胞是牙周炎中破坏牙齿骨骼的细胞。我们预计,我们的研究将产生可用于治疗干预的见解,以抑制与牙周炎相关的骨和牙齿破坏。

项目成果

期刊论文数量(0)
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Lionel B Ivashkiv其他文献

Lionel B Ivashkiv的其他文献

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{{ truncateString('Lionel B Ivashkiv', 18)}}的其他基金

Negative Regulation of Osteoclastogenesis
破骨细胞生成的负调控
  • 批准号:
    8369428
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Negative Regulation of Osteoclastogenesis
破骨细胞生成的负调控
  • 批准号:
    8685764
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Negative Regulation of Osteoclastogenesis
破骨细胞生成的负调控
  • 批准号:
    8481532
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Negative Regulation of Osteoclastogenesis
破骨细胞生成的负调控
  • 批准号:
    10112883
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Negative Regulation of Osteoclastogenesis
破骨细胞生成的负调控
  • 批准号:
    10372951
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Negative Regulation of Human Osteoclastogenesis
人类破骨细胞生成的负调控
  • 批准号:
    7684207
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Negative Regulation of Human Osteoclastogenesis
人类破骨细胞生成的负调控
  • 批准号:
    7555286
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Negative Regulation of Human Osteoclastogenesis
人类破骨细胞生成的负调控
  • 批准号:
    7882459
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Negative Regulation of Osteoclastogenesis
破骨细胞生成的负调控
  • 批准号:
    9089604
  • 财政年份:
    2008
  • 资助金额:
    $ 42.01万
  • 项目类别:
Interferon Regulation in Systemic Lupus
系统性狼疮中的干扰素调节
  • 批准号:
    7183510
  • 财政年份:
    2006
  • 资助金额:
    $ 42.01万
  • 项目类别:

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