THE CART RECEPTOR

购物车接收器

• 批准号: 8172450
• 负责人: MICHAEL J KUHAR
• 金额: $ 2.74万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172450
• 关键词: Binding; Biological Assay; Cell Line; Cells; Cloning; Computer Retrieval of Information on Scientific Projects Database; Funding; Goals; Grant; Institution; Measures; Microarray Analysis; Orphan; PC12 Cells; Peptide Receptor; Pharmaceutical Preparations; Publications; Research; Research Personnel; Resources; Screening procedure; Source; System; United States National Institutes of Health; Work; cocaine- and amphetamine-regulated transcript protein; improved; receptor; receptor binding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The last major part of the CART system to be identified is the CART receptor (it is possible that there are several receptors). Screening for medications related to CART peptides requires an ability to work with and express the receptor. While there have been several publications on the receptor, we have begun an intensive study with the goal of improving assays and a cloning of the receptor. The CART receptor binding assay has been standardized, and levels of specific binding in several cell lines have been measured. Specifically specific binding has been found in PC12 cells; when these cells differentiate, the binding increases. Microarray analysis has identified several possible receptors that are increased after differentiation and are therefore receptor candidates. Another approach to finding the CART peptide receptor has been to study known orphan receptors (an orphan is a receptor that is known, but whose activating substrate is unknown). Some orphan receptors have been expressed in cells, but as of this date, it is not yet clear if these are candidates. We continue to make significant progress identifying the CART peptide receptor(s).

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 CART系统的最后一个主要部分是CART受体（可能有几种受体）。 筛选与CART肽相关的药物需要与受体一起工作并表达受体的能力。 虽然已经有几篇关于受体的出版物，但我们已经开始了深入的研究，目的是改进受体的测定和克隆。 CART受体结合试验已经标准化，并且已经测量了几种细胞系中的特异性结合水平。 在PC 12细胞中发现了特异性结合;当这些细胞分化时，结合增加。 微阵列分析已经确定了几种可能的受体，它们在分化后增加，因此是受体候选者。 寻找CART肽受体的另一种方法是研究已知的孤儿受体（孤儿受体是已知的受体，但其激活底物是未知的）。 一些孤儿受体已在细胞中表达，但截至目前，尚不清楚这些是否是候选者。 我们继续在鉴定CART肽受体方面取得重大进展。