PROMOTER CHARACTERIZATION OF THE CART GENE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 大多数对CART多肽的研究都涉及到将多肽注射到大脑中并研究其效果。因此，启动了这一旨在减少内生性CART的补充项目。已经发现，在初步实验中，有几个siRNA可以降低CART多肽水平。此外，还进行了长期表达siRNA的病毒颗粒的注射。在CART多肽水平降低的动物身上，正在进行有用的行为研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Most studies of CART peptides involve injecting the peptide into the brain and studying its effects. Therefore, this complementary project aimed at reducing endogenous CART was initiated. Several siRNAs have been found that, in preliminary experiments, reduce CART peptide levels. Also, injection of viral particles that will express siRNAs long term has been carried out. Useful behavioral studies are being carried out in animals where CART peptide levels have been reduced.