DEVELOPMENT OF GENOMIC RESOURCES FOR THE PRAIRIE VOLE

草原田鼠基因组资源的开发

• 批准号: 8172429
• 负责人: Larry J Young
• 金额: $ 4.39万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172429
• 关键词: Chromosome Mapping; Communities; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Resequencing; Deposition; Development; Funding; Genbank; Genes; Genetic; Genome; Genomics; Grant; Individual; Institution; Libraries; Maps; Microtus; Reporting; Research; Research Personnel; Resources; Source; United States National Institutes of Health; prairie vole

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the reporting period, we continued with the creation of the 6x BAC library representing the prairie vole genome. Using this BAC library, we isolated and fully sequenced BAC clones containing 19 genes that are currently being used in the prairie vole research community. We mapped 70 of these BACs to chromosomal regions using FISH. In addition, we collected DNA from over 300 individual prairie voles of known lineage which will be used to create the linkage map. These sequences have been deposited in Genbank. We have end sequenced 256 random BAC clones and by resequencing these loci in 3 additional voles we have identified 1000 SNPs in more than 300 genetic loci.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 报告期内，我们继续创建代表草原田鼠基因组的6x BAC文库。 使用此 BAC 文库，我们分离并完全测序了包含 19 个基因的 BAC 克隆，这些基因目前用于 草原田鼠研究社区。 我们使用 FISH 将其中 70 个 BAC 映射到染色体区域。 此外，我们还从 300 多只已知谱系的草原田鼠个体中收集了 DNA，这些 DNA 将用于创建连锁图谱。这些序列已存入Genbank。 我们对 256 个随机 BAC 克隆进行了最终测序，并通过对另外 3 个田鼠中的这些基因座进行重新测序，我们在 300 多个基因位点中鉴定了 1000 个 SNP。