DNA MICROARRAY AND EXPRESSION CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 TNPRC的DNA微阵列和表达核心(微阵列核心)目前提供以下服务：a.DNA微阵列：1)微阵列实验设计；2)斑点或双色微阵列实验；3)微阵列数据分析；4)小样本扩增；5)聚合酶链式反应阵列；6)RNA分离。B.基于siRNA的基因沉默：1)设计和合成对TNPRC社区有用的siRNA子集；2)个体siRNA检测的优化和标准化。 在2009年期间，该核心为不同的研究人员进行了大约150次微阵列杂交。总共积累了1.0太字节的原始数据。与前一年大多数是结核分枝杆菌阵列形成对比的是，今年的大部分工作是宿主阵列，包括恒河猴微阵列()、人类微阵列(12)和哺乳动物miRNA微阵列(20)。产生的一些数据已经导致出版物(8份出版物)，而目前正在分析更多的数据。去年在核心活动中又增加了一项服务：miRNA阵列。这项服务非常受欢迎，已经帮助提交了两份赠款申请，其中一份已经成功，另一份仍在等待中。 在接下来的几年里，核心希望扩展到定制的微阵列。第一批将由CORE设计、制造和生产的阵列将包括恒河猴组织阵列(肠道、大脑)、泛猕猴淋巴芯片和用于监测“无病原体”猕猴群体的多病原体检测芯片。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DNA Microarray and Expression Core (Microarray Core) at TNPRC currently provides the following services: A. DNA Microarrays: 1) Microarray experimental design; 2) Spotted or 2-color microarray experiments; 3) Microarray Data Analysis; 4) Small Sample Amplification; 5) PCR Arrays; and 6) RNA Isolation. B. siRNA based gene-silencing: 1) Design and synthesis of a subset of siRNA's useful to the TNPRC community; 2) Optimization and standardization of individual siRNA assay. During the year 2009, the core performed approximately 150 microarray hybridizations for various investigators. In all 1.0 Terabytes of raw data was accumulated. In contrast to the prior year when the majority were M. tuberculosis arrays this year the majority of the work were host arrays, including rhesus macaque microarrays (64), human microarrays (12) and mammalian miRNA microarrays (20). Some of the data generated has already lead to publications (8 publications), while much more is currently being analyzed. One additional service was added to the core activities in the last year: miRNA array. This service has been very popular, and has already contributed to the submission of two grant applications, of which one has been successful, while the other remains pending. In the coming years, the core would like to expand into custom microarrays. The first few arrays that will be designed, fabricated and produced by the core will include rhesus macaque tissue arrays (Gut, brain), a pan-macaque lympho-chip and a multi-pathogen detection chip for surveillance of the "pathogen-free" macaque colony.