ALCOHOL AND TB IN NONHUMAN PRIMATES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 翻译后摘要：结核分枝杆菌（Mtb）感染全球20亿人。酗酒者比正常宿主有更大的结核病负担，酒精会阻碍疾病的进展和结果。非人灵长类动物最好地概括了人类TB综合征，并且TNPRC的Kaushal博士的小组已经在暴露于雾化Mtb的猕猴中产生了人类TB模型[Dutta等人，J. Infect Dis，2010，201：]。此外，新奥尔良路易斯安那州立大学医学院的综合酒精研究中心（CARC）在研究非人灵长类动物酗酒模型中的并发传染病方面具有相当丰富的专业知识。 我们假设，恒河猴酗酒消费将产生不利影响遏制毒性结核分枝杆菌通过改变肺CD 4 + Th 1效应细胞的数量和功能，导致感染的加速进展。我们提出以下具体目标： 1. 目的评价嗜酒精猕猴肺结核分枝杆菌强毒感染的清除效果。 2. 通过转录组学表征这些动物的免疫反应以及基因表达。 该项目已获得资金，预计一旦有适当的BSL-3空间，就将开始。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Abstract: Mycobacterium tuberculosis (Mtb) infects two billion people worldwide. Alcoholics have a greater burden of TB than normal hosts, and alcohol worsens the progression and the outcome of the disease. Nonhuman primates best recapitulate the human TB syndrome, and Dr. Kaushal's group at the TNPRC has generated a model of human TB in macaques exposed to aerosolized Mtb [Dutta et al, J. Infect Dis, 2010, 201:]. Further, the Comprehensive Alcohol Research Center (CARC) at the LSU School of Medicine, New Orleans has considerable expertise in studying co-incident infectious diseases in a nonhuman primate model of binge alcoholism. We hypothesize that binge alcohol consumption by rhesus macaques will adversely affect the containment of virulent Mtb via alterations in the lung CD4+ Th1 effector cell number and function, causing accelerated progression of infection. We propose the following specific aims: 1. To evaluate the mycobacterial clearance of pulmonary infection with virulent Mtb in alcohol consuming rhesus monkeys. 2. Characterize the immune response in these animals as well as gene expression through transcriptomics. This project has been funded, and is expected to start as soon as appropriate BSL-3 space is available.