PRIMATE GENETIC ANALYSIS AND PEDIGREE MANAGEMENT

灵长类动物遗传分析和谱系管理

• 批准号: 8172351
• 负责人: JAMES GIBSON ELSE
• 金额: $ 10.97万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172351
• 关键词: Acquired Immunodeficiency Syndrome; Animals; Birth; Breeding; Cercocebus; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; DNA; Data; Data Set; Databases; Funding; Genetic; Genetic Markers; Genotype; Grant; Institution; Macaca; Macaca mulatta; Microsatellite Repeats; Primates; Records; Research; Research Personnel; Resources; Sampling; Source; System; United States National Institutes of Health; Veterinarians; cohort; genetic analysis; genetic pedigree; meetings; trait

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Samples for DNA extraction to determine parentage have been collected from 1,409 rhesus macaque comprising the Yerkes' breeding colony of 1,691. This includes 614 animals from the SPF colonies dedicated to AIDS research, and 795 animals from NSPF animals. Each of these samples has been genotyped for 19 highly polymorphic microsatellites spread throughout the autosomal chromosomes. In addition 282 samples from the 2008 birth cohort and 462 samples from the 2009 birth cohort have been collected, and genotyping efforts are currently underway for these samples. These data are used to determine parentage, pedigree, and selected genetic markers for all of our macaques and mangabeys maintained at the Field Station. The ability to characterize specific genetic components has enabled us to better meet specific investigator needs, develop more diverse research endeavors, to selectively breed for specific genetic traits, and to undertake specific phenotypic comparisons. To manage this data set, we are establishing a full-scale database system that will be able to assimilate genetic, parentage, pedigree, and demographic variables on all the animals. This database system will be incorporated into our current animal records system, which will enable Yerkes' investigators and veterinarians to track, manage and view an animal's record with a single query.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 从1，409只恒河猴中收集了用于DNA提取以确定亲子关系的样本，其中包括1，691只耶基斯繁殖群。 这包括614只来自致力于艾滋病研究的SPF动物群和795只来自NSPF动物群的动物。这些样本中的每一个都被基因分型为遍布常染色体的19个高度多态性微卫星。此外，从2008年出生队列中收集了282份样本，从2009年出生队列中收集了462份样本，目前正在对这些样本进行基因分型。 这些数据被用来确定亲子关系，谱系，并选择遗传标记为我们所有的猕猴和白眉猴保持在外地站。 表征特定遗传组分的能力使我们能够更好地满足特定研究者的需求，开展更多样化的研究工作，选择性地培育特定的遗传性状，并进行特定的表型比较。 为了管理这个数据集，我们正在建立一个全面的数据库系统，该系统将能够吸收所有动物的遗传，亲子关系，谱系和人口统计学变量。 这个数据库系统将被纳入我们目前的动物记录系统，这将使耶基斯的调查人员和兽医跟踪，管理和查看动物的记录与一个单一的查询。