PRIMATE GENETIC ANALYSIS AND PEDIGREE MANAGEMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 用于提取DNA以确定亲子关系的样本已经从1409只恒河猴身上采集，其中包括1691只耶克猕猴的繁殖群体。这包括来自SPF群体的614只致力于艾滋病研究的动物，以及来自NSPF动物的795只动物。这些样本中的每一个都对分布在常染色体上的19个高度多态的微卫星进行了基因分型。此外，还收集了2008年出生队列的282个样本和2009年出生队列的462个样本，目前正在对这些样本进行基因分型工作。 这些数据被用来确定我们在野外空间站维护的所有猕猴和黑尾猴的亲缘关系、系谱和选定的遗传标记。表征特定遗传成分的能力使我们能够更好地满足特定的研究人员的需求，开展更多样化的研究工作，有选择地为特定的遗传特征进行繁殖，并进行特定的表型比较。 为了管理这个数据集，我们正在建立一个全面的数据库系统，它将能够同化所有动物的遗传、亲子关系、系谱和人口统计变量。这个数据库系统将被整合到我们目前的动物记录系统中，这将使耶克斯的调查人员和兽医能够通过一个查询来跟踪、管理和查看动物的记录。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Samples for DNA extraction to determine parentage have been collected from 1,409 rhesus macaque comprising the Yerkes' breeding colony of 1,691. This includes 614 animals from the SPF colonies dedicated to AIDS research, and 795 animals from NSPF animals. Each of these samples has been genotyped for 19 highly polymorphic microsatellites spread throughout the autosomal chromosomes. In addition 282 samples from the 2008 birth cohort and 462 samples from the 2009 birth cohort have been collected, and genotyping efforts are currently underway for these samples. These data are used to determine parentage, pedigree, and selected genetic markers for all of our macaques and mangabeys maintained at the Field Station. The ability to characterize specific genetic components has enabled us to better meet specific investigator needs, develop more diverse research endeavors, to selectively breed for specific genetic traits, and to undertake specific phenotypic comparisons. To manage this data set, we are establishing a full-scale database system that will be able to assimilate genetic, parentage, pedigree, and demographic variables on all the animals. This database system will be incorporated into our current animal records system, which will enable Yerkes' investigators and veterinarians to track, manage and view an animal's record with a single query.