SPF BREEDING COLONIES-SUPPLEMENT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这是SPF繁殖群体补充剂。动物可以作为NIH支持的艾滋病相关研究的研究人员的研究对象，并为国家卫生优先事项做出贡献。这个项目的目标是获得关于遗传MHC区域的更完整的信息，这对耶克斯灵长类中心的所有SPF猕猴来说是至关重要的。这些信息将大大提高这些动物在该中心进行的所有传染病研究中的效用。这包括MHC区域的单倍型分析和该区域内9个特定功能等位基因的分型。到目前为止，我们已经收集了动物的血液样本进行分析，目前正在进行所有必要的DNA提取。优化了每个微卫星的聚合酶链式反应条件，设计了多标记板。对人口中的一小部分进行了初步分析，以确保取得预期结果。目前正在进行特定功能等位基因分型的优化，希望一旦DNA提取完成，就能同时执行这一项目的两个方面。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This is the SPF Breeding Colony Supplement. Animals are made available as subjects to NIH supported investigators for AIDS related research and contribute to national health priorities. The goal of this project is to obtain more complete information regarding the genetic MHC region, which is vital for all SPF rhesus macaques housed at the Yerkes Primate Center. This information will greatly enhance the utility of these animals for all infectious disease studies undertaken at the Center. This includes haplotype analysis of the MHC region and typing of nine specific functional alleles within the region. To date, we have collected blood samples from the animals for analysis and are currently performing all the necessary DNA extractions. PCR conditions for each microsatellite have been optimized and multimarker panels designed. Preliminary analysis of a small subset of the population has been performed to ensure that expected results were obtained. Specific functional allele typing is currently undergoing optimization with the expectation to perform both aspects of this project as soon as DNA extractions are complete.