SPF BREEDING COLONIES-SUPPLEMENT

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 这是SPF繁殖群补充剂。 动物可作为受试者提供给NIH支持的研究人员进行艾滋病相关研究，并为国家卫生优先事项做出贡献。 该项目的目标是获得关于遗传MHC区域的更完整的信息，这对Yerkes灵长类动物中心的所有SPF恒河猴至关重要。 这些信息将大大提高这些动物在中心进行的所有传染病研究中的效用。 这包括MHC区域的单倍型分析和该区域内9个特异性功能等位基因的分型。 到目前为止，我们已经收集了动物的血液样本进行分析，目前正在进行所有必要的DNA提取。 每个微卫星的PCR条件进行了优化和多标记面板的设计。 对一小部分人群进行了初步分析，以确保获得预期结果。 特定的功能等位基因分型目前正在进行优化，期望在DNA提取完成后立即执行该项目的两个方面。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is the SPF Breeding Colony supplement. Animals are made available as subjects to NIH supported investigators for AIDS related research and contribute to national health priorities. The goal of this project is to obtain more complete information regarding the genetic MHC region, which is vital for all SPF rhesus macaques housed at the Yerkes Primate Center. This information will greatly enhance the utility of these animals for all infectious disease studies undertaken at the Center. This includes haplotype analysis of the MHC region and typing of nine specific fuctional alleles within the region. To date, we have collected blood samples from the animals for analysis and are currently performing all the necessary DNA extractions. PCR conditions for each microsatellite have been optimized and multimarker panels designed. Preliminary analysis of a small subset of the population has been performed to ensure expected results were obtained. Specific functional allele typing is currently undergoing optimization with the expectation to perform both aspects of this project as soon as DNA extractions are complete.