SPF BREEDING COLONIES AT THE YERKES NPRC: AIDS, ANIMAL MODEL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 描述(由申请人提供)：本项目的中心目标是培育无特定病原体(SPF-由RFA RR-G2-005中的NCRR定义)、MHC分型和基因特征的恒河猴，为NIH支持的艾滋病相关研究人员提供研究对象。这一目标将在YNPRC国家灵长类研究中心实现，利用现有的745只SPF猕猴种群(自2002年以来在NCRR合作协议U24 RR 018109的支持下培育和维护)，并雇用熟练和经验丰富的人员以及迄今非常成功的经过验证的方法和程序。现有的SPF恒河猴群体将被管理以优化繁殖，既为艾滋病研究人员提供研究对象，又通过繁殖增加其总规模。此外，在这个项目阶段的头两年，我们将通过适当的测试和分离，继续从YNPRC非SPF繁殖群体招募幼崽。目标是确保提供SPF受试者，以满足埃默里以及来自区域和国家其他机构的艾滋病调查人员的要求；这是我们在过去的颁奖期间(2002年至2007年)非常成功地实现的目标。YNPRC的总体目标是到2013年过渡到完全以SPF野战站为基础的恒河猴群体，逐步用SPF动物取代该群体中的非SPF恒河猴。这一目标虽然在科学和安全方面对中心(和NCRR)的利益至关重要，但超出了本提案的范围。因此，我们在这里寻求对500只SPF猴子的固定群体的支持，尽管我们描述了一开始包括745只动物的计划，并且我们打算在拟议的获奖期内大幅增加。这一机制将支持的500个群体的建议与最初获奖期间的努力持平，加上我们筛查计划中的补充动物，足以满足我们的艾滋病研究组合的要求。超过500只的SPF动物将从NCRR向YNPRC(P51-RR00165)提供的基础补助金(例如，按日和使用费)相关的计划收入中获得支持。随着SPF群体的增长，传统群体的规模将会缩小，目标是到2013年实现100%SPF恒河猴繁殖群体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The central objective of this project is to breed rhesus macaques that are specific pathogen free (SPF- as defined by NCRR in RFA RR-G2-005), MHC typed and genetically characterized to provide research subjects to NIH supported investigators for AIDS related research. This objective will be achieved at the YNPRC National Primate Research Center (YNPRC) utilizing an existing colony of 745 SPF macaque monkeys (produced and maintained since 2002 with the support of NCRR Cooperative Agreement U24 RR 018109) and employing skilled and experienced personnel and proven methods and procedures that have been highly successful to date. The existing SPF rhesus macaque colony will be managed to optimize reproduction, both to provide subjects to AIDS investigators, and to increase its total size via breeding. Additionally, during the first two years of this project phase, we will continue recruitment of young animals from YNPRC non-SPF breeding colonies via appropriate testing and separation. The goal is to ensure the availability of SPF subjects to meet the requirements of AIDS investigators, both Emory based and from other institutions regionally and nationally; this is an objective we have achieved very successfully in the past award period (2002 to 2007). The overall YNPRC goal is to transition to an entirely SPF Field Station based rhesus colony by 2013, progressively replacing the non-SPF rhesus macaques in the colony with SPF animals. This goal, although vital to the interests of the Center (and NCRR) in both scientific and safety terms, goes beyond the scope of the present proposal. Thus, we seek here support for a fixed colony of 500 SPF monkeys although we describe a program that includes 745 animals at the outset and that we intend to increase substantially in the proposed award period. The proposed colony of 500 to be supported by this mechanism is level with the effort in the initial award period and, with supplemental animals from our screening program, is sufficient to meet the requirements of our AIDS research portfolio. The SPF animals in excess of the 500 will be supported from program income associated with the base grant (e.g. per diem and use fees) from NCRR to the YNPRC (P51 - RR00165). As the SPF colony grows the conventional colony will be reduced in size, with the goal of achieving a 100% SPF rhesus breeding colony by 2013.