PRIMATE GENETIC ANALYSIS AND PEDIGREE MANAGEMENT

灵长类动物遗传分析和谱系管理

• 批准号: 8357419
• 负责人: JAMES GIBSON ELSE
• 金额: $ 7.43万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357419
• 关键词: Acquired Immunodeficiency Syndrome; Animal Experimentation; Animals; Breeding; Cercocebus; Chromosomes; DNA; Data; Data Set; Databases; Funding; Genetic; Genetic Markers; Genotype; Grant; Macaca; Macaca mulatta; Microsatellite Repeats; National Center for Research Resources; Primates; Principal Investigator; Records; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Source; System; United States National Institutes of Health; Veterinarians; cost; genetic analysis; genetic pedigree; meetings; trait

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Samples for DNA extraction to determine parentage have been collected from over 2000 rhesus macaque, comprising the Yerkes' breeding colony. This includes animals from both the SPF colonies dedicated to AIDS research, and the animals from the NSPF animals. Each of these samples has been genotyped for 19 highly polymorphic microsatellites spread throughout the authosomal chromosomes. These data are used to determine parentage, pedigree, and selected genetic markers for all of our macaques and mangabeys maintained at the Field Station. The ability to characterize specific genetic components has enabled us to better meet specific investigator needs, to develop more diverse research endeavors, to selectively breed for specific genetic traits, and to undertake specific phenotypic comparisons. To manage this data set, we are establishing a full-scale database system that will be able to assimilate genetic, parentage, pedigree, and demographic variable on all the animals. This database system will be incorporated into our current animal records system, which will enable Yerkes' investigators and veterinarians to track, manage, and view an animal's record in a single query.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 从2000多只恒河猴中收集了DNA提取样本，以确定亲子关系，包括耶基斯的繁殖群。这包括来自致力于艾滋病研究的SPF群体的动物，以及来自NSPF动物的动物。这些样品中的每一个都已被基因分型为19个高度多态性的微卫星分布在整个真体染色体。这些数据被用来确定亲子关系，谱系，并选择遗传标记为我们所有的猕猴和白眉猴保持在外地站。表征特定遗传组分的能力使我们能够更好地满足特定研究者的需求，开展更多样化的研究工作，选择性地培育特定的遗传性状，并进行特定的表型比较。为了管理这个数据集，我们正在建立一个全面的数据库系统，该系统将能够吸收所有动物的遗传，亲子关系，谱系和人口统计学变量。这个数据库系统将被纳入我们目前的动物记录系统，这将使耶基斯的研究人员和兽医跟踪，管理和查看动物的记录在一个单一的查询。