ESTABLISHMENT & MAINTENANCE OF A CLOSED CPRC SPF COLONY

机构

• 批准号: 8356896
• 负责人: Edmundo Nelson Kraiselburd
• 金额: $ 112.48万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8356896
• 关键词: Acquired Immunodeficiency Syndrome; Animals; Breeding; Caribbean region; Funding; Genetic Heterogeneity; Genetic Markers; Grant; Housing; Macaca; Macaca mulatta; Maintenance; Monkeys; National Center for Research Resources; Papovaviridae; Primates; Principal Investigator; Reproduction; Research; Research Infrastructure; Resources; SIV; Simian T-lymphotropic virus 1; Simian virus 40; Source; Specific Pathogen Frees; Structure; Testing; United States National Institutes of Health; Virus; cost; germ free condition; meetings; offspring; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DESCRIPTION (provided by applicant): The objective of this proposal is to maintain a closed specific pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station of the Caribbean Primate Research Center (CPRC). The founding stock of genetically-characterized Indian-origin monkeys donated by CPRC will consist of approximately 755 SPF (Herpes B, STLV-1, SIV and SRV-free) rhesus macaques of which 164 (or 22%) will also be free of the papova virus SV40. Thus, this colony will consist of two SPF sub colonies: one SV40-free and the other SV40 positive. Animals from these two closed SPF sub colonies will be housed in reproduction corncribs, and will be quarterly tested for the presence of the above mentioned target viruses. A panel of highly polymorphic genetic markers will be used to characterize the colony and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. By year five, the program will produce a total estimated offspring of 1,082 SPF rhesus macaques, of which 301 will be also SV40 negative. These SPF macaques will help meet the increased research demand for these animals. At the end of this 5 year grant, it is expected that this program will produce more than 160 animals/year and will attain self-sufficiency. CPRC will continue to make significant contributions to AIDS and to other research programs by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research initiatives.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 描述(由申请人提供)：本提案的目标是在加勒比灵长类动物研究中心(CPRC)的Sabana Seca野外站维持一个封闭的无特定病原体(SPF)猕猴供应和繁殖群体计划。CPRC捐赠的具有基因特征的印度原产猴子的创始种群将由大约755只SPF(B疱疹B、STLV-1、SIV和SRV-Free)恒河猴组成，其中164只(或22%)也将没有Papova病毒SV40。因此，这个菌落将由两个SPF亚克隆组成：一个不含SV40，另一个呈SV40阳性。来自这两个封闭SPF亚群的动物将被安置在繁殖玉米床中，并将每季度进行一次上述目标病毒的检测。将使用一组高度多态的遗传标记来描述群体特征并优化繁育群体的结构，最大限度地提高其遗传异质性水平。到第五年，该计划将产生总计1082只SPF恒河猴后代，其中301只也将是SV40阴性。这些SPF猕猴将有助于满足对这些动物日益增长的研究需求。在这项为期5年的赠款结束时，预计该计划每年将生产160多只动物，并将实现自给自足。CPRC将继续为艾滋病和其他研究项目做出重大贡献，为NIH赞助的研究项目提供高质量和健康的SPF恒河猴。