CARIBBEAN PRIMATE RES CTR SPF RHESUS MONKEY PROGRAM: HIV ANIMAL MODELS

加勒比灵长类动物 RES CTR SPF 恒河猴项目：HIV 动物模型

• 批准号: 8173539
• 负责人: Edmundo Nelson Kraiselburd
• 金额: $ 18.54万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173539
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Animals; Biomedical Research; Breeding; Caribbean region; Cercopithecine Herpesvirus 1; Computer Retrieval of Information on Scientific Projects Database; Female; Funding; Future; Grant; HIV; Herpesviridae; Institution; Island; Macaca; Macaca mulatta; Maintenance; Monkeys; Population; Primates; Production; Research; Research Personnel; Resources; Retroviridae; SIV; Simian T-lymphotropic virus 1; Simian virus 40; Source; Specific Pathogen Frees; Surveys; United States National Institutes of Health; Virus; germ free condition; immature animal; male; man; meetings; offspring; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regarding HIV. The objective of this proposal is to establish and maintain a specific-pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station (SSFS) of the Caribbean Prima Research Center (CPRC). This program will use genetically-characterized, MHC- typed, Indian-origin monkeys from the CPRC''s free-ranging colony on the island of Cayo Santiago (CS). The CPRC will donate the founding stock for the colony from its Cayo Santiago and Cayo Santiago-derived populations. Previous surveys have shown that the CS macaques are free of several important viruses including retroviruses and simian virus 40 (SV-40), and that the majority of immature animals are negative for B-virus (Herpesvirus simiae Cercopithecine herpesvirus type 1). About 20- 25% of these monkeys are also Mamu-A*01 positive. Currently there is a shortage of SPF rhesus monkeys for biomedical research and the demand for these animals expected to increase dramatically in the future. The establishment and maintenance of this program at 551 will help meet the increased demand for both SPF (B-virus, SRV-D, SIV, STLV-1 and SV404ee) and Man'' A*01 positive rhesus monkeys. Forty-five SPF females and nine SPF males (nine breeding groups) will added to the colony each year through internal recruitment from CS and SSFS. MHC-typing and selective breeding will be used to increase the production of SPF Mamu- A*01 positive offspring. By year five t program will have supplied 106 offspring including about 30 Mamu-A*01 positive animals. By year 6 or 7, the program should be self-supporting. The CPRC''s program will make a significant contribution to advancing AIDS research by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research programs.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 关于艾滋病毒。该提案的目标是在加勒比初级研究中心 (CPRC) 的萨巴纳塞卡野外站 (SSFS) 建立和维持无特定病原体 (SPF) 恒河猴供应和繁殖群计划。该项目将使用来自 CPRC 在圣地亚哥岛 (CS) 自由放养猴群的具有遗传特征、MHC 类型、印度血统的猴子。 CPRC 将从圣地亚哥岛和圣地亚哥岛衍生的种群中捐赠该殖民地的创始种群。之前的调查显示，CS猕猴没有携带逆转录病毒和猿猴病毒40（SV-40）等多种重要病毒，并且大多数未成熟动物的B病毒（猴疱疹病毒1型）呈阴性。这些猴子中大约 20-25% 也是 Mamu-A*01 阳性。目前用于生物医学研究的 SPF 恒河猴短缺，预计未来对这些动物的需求将急剧增加。 551 项目的建立和维护将有助于满足对 SPF（B 病毒、SRV-D、SIV、STLV-1 和 SV404ee）和 Man'' A*01 阳性恒河猴不断增长的需求。每年，通过 CS 和 SSFS 的内部招募，将有 45 只 SPF 雌性和 9 只 SPF 雄性（九个繁殖群体）加入该群体。 MHC 分型和选择性育种将用于增加 SPF Mamu-A*01 阳性后代的产量。到第五年，t 计划将提供 106 只后代，其中包括约 30 只 Mamu-A*01 阳性动物。到第六年或第七年，该计划应该是自负盈亏的。 CPRC 的项目将为 NIH 资助的研究项目提供高质量、健康的 SPF 恒河猴，为推进艾滋病研究做出重大贡献。