CARIBBEAN PRIMATE RES CTR SPF RHESUS MONKEY PROGRAM: HIV THERAPEUTIC AGENT DVMT

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 关于艾滋病毒，该提案是在加勒比海Prima研究中心（CPRC）的Sabana Seca野外站（SSFS）建立和维持一个无特定病原体（SPF）恒河猴供应和繁殖群计划。该项目将使用来自CPRC在Cayo圣地亚哥岛（CS）的自由放养群的具有遗传特征的、MHC分型的印度源猴。CPRC将从圣地亚哥岛和圣地亚哥岛衍生的种群中为殖民地捐赠创始种群。先前的调查表明，CS猕猴没有几种重要的病毒，包括逆转录病毒和猿猴病毒40（SV-40），大多数未成年动物对B病毒（猿猴疱疹病毒1型）呈阴性。这些猴子中约有20- 25%也是Mamu-A*01阳性。目前，用于生物医学研究的SPF恒河猴短缺，预计未来对这些动物的需求将大幅增加。在551建立和维持该项目将有助于满足SPF（B病毒、SRV-D、SIV、STLV-1和SV 404 ee）和Man' A*01阳性恒河猴的需求增加。每年从CS和SSFS内部招募45只SPF雌性和9只SPF雄性（9个繁殖组）加入殖民地。将使用MHC分型和选择性育种来增加SPF Mamu-A *01阳性后代的产量。到第五年，t计划将提供106只后代，包括约30只Mamu-A*01阳性动物。到第六年或第七年，该计划应该是自给自足的。CPRC的计划将通过为NIH赞助的研究计划提供高质量和健康的SPF恒河猴，为推进艾滋病研究做出重大贡献。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. REGARDING HIV this proposal is to establish and maintain a specific-pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station (SSFS) of the Caribbean Prima Research Center (CPRC). This program will use genetically-characterized, MHC- typed, Indian-origin monkeys from the CPRC's free-ranging colony on the island of Cayo Santiago (CS). The CPRC will donate the founding stock for the colony from its Cayo Santiago and Cayo Santiago-derived populations. Previous surveys have shown that the CS macaques are free of several important viruses including retroviruses and simian virus 40 (SV-40), and that the majority of immature animals are negative for B-virus (Herpesvirus simiae Cercopithecine herpesvirus type 1). About 20- 25% of these monkeys are also Mamu-A*01 positive. Currently there is a shortage of SPF rhesus monkeys for biomedical research and the demand for these animals expected to increase dramatically in the future. The establishment and maintenance of this program at 551 will help meet the increased demand for both SPF (B-virus, SRV-D, SIV, STLV-1 and SV404ee) and Man' A*01 positive rhesus monkeys. Forty-five SPF females and nine SPF males (nine breeding groups) will added to the colony each year through internal recruitment from CS and SSFS. MHC-typing and selective breeding will be used to increase the production of SPF Mamu- A*01 positive offspring. By year five t program will have supplied 106 offspring including about 30 Mamu-A*01 positive animals. By year 6 or 7, the program should be self-supporting. The CPRC's program will make a significant contribution to advancing AIDS research by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research programs.