ENHANCEMENT OF THE CPRC-SPF RHESUS MONKEY PROGRAM: AIDS GENOME

描述（由申请人提供）：本提案的目的是加强现有的SPF恒河猴供应和繁殖种群计划。该计划将使用从CPRC在CS岛的自由放养殖民地获得的已建立社会群体中获得的具有遗传特征的主要组织相容性复合体（MHC）型印度裔猴子。CPRC将为该群体捐赠创始种群，该群体由102只成年雌性和21只幼年雌性以及至少20只雄性组成。以前的调查表明，圣地亚哥群岛猕猴不含几种重要的病毒，包括逆转录病毒，而且大多数未成熟的动物对B病毒呈阴性。这些猴子中约有20-25%也是Mamu-A*01阳性。目前，用于生物医学研究的SPF恒河猴短缺，预计未来对这些动物的需求将急剧增加。该项目的建立和维持将有助于满足对SPF （B病毒、SRV-D病毒、SIV病毒和无stlv -l病毒）和Mamu-A*01阳性恒河猴日益增长的需求。mhc分型和选择性育种将用于增加SPF Mamu-A*01阳性后代的产量。预计在第一年，创始队列将产生至少70只婴儿，并在五年内估计总共产生约400只SPF动物的后代。到第6年，该项目应该能够自给自足。CPRC的项目将为推进生物医学研究，特别是艾滋病研究做出重大贡献，为nih赞助的研究项目提供高质量和健康的SPF恒河猴。

DESCRIPTION (Provided by applicant): The objective of this proposal is to enhance the existing SPF rhesus macaque supply and breeding colony program at the SSFS of the CPRC. This program will use genetically characterized, major histocompatability complex (MHC)-typed, Indian-origin monkeys from established social groups obtained from the CPRC's free-ranging colony on the island of CS. The CPRC will donate the founding stock for the colony, which consists of 102 adult and 21 juvenile females and minimum of 20 sires. Previous surveys have shown that the Cayo Santiago macaques are free of several important viruses including retroviruses, and that the majority of immature animals are negative for B virus. About 20-25% of these monkeys are also Mamu-A*01 positive. Currently, there is a shortage of SPF rhesus monkeys for biomedical research and the demand for these animals is expected to increase dramatically in the future. The establishment and maintenance of this expanded program at SSFS will help meet the increased demand for both SPF (B virus, SRV-D, SIV, and STLV-l-free) and Mamu-A*01 positive rhesus monkeys. MHC-typing and selective breeding will be used to increase the production of SPF Mamu-A*01 positive offspring. The founding cohort is expected to produce a minimum of 70 infants during the first year and an estimated total offspring of about 400 SPF animals in five years. By Year 6 the program should be self-supporting. The CPRC's program will make a significant contribution to advancing biomedical research, and in particular to AIDS research by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research programs.