ENHANCEMENT OF THE CPRC-SPF RHESUS MONKEY PROGRAM: AIDS IMMUNOLOGY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 描述（由申请人提供）：本提案的目的是加强CPRC SSFS现有的SPF恒河猴供应和繁殖群计划。 该计划将使用遗传特征，主要组织相容性复合体（MHC）型，印度起源的猴子从建立的社会群体获得的CPRC的自由放养殖民地在CS岛。 CPRC将为殖民地捐赠基础种群，该种群由102只成年雌性和21只幼年雌性以及至少20只公畜组成。 以前的调查表明，圣地亚哥岛猕猴没有几种重要的病毒，包括逆转录病毒，大多数未成年动物对B病毒呈阴性。 这些猴子中约有20-25%也是Mamu-A*01阳性。 目前，用于生物医学研究的SPF恒河猴短缺，预计未来对这些动物的需求将大幅增加。 在SSFS建立和维持这一扩大的项目将有助于满足SPF（无B病毒、SRV-D、SIV和STLV-1）和Mamu-A*01阳性恒河猴的需求。 将使用MHC分型和选择性育种来增加SPF Mamu-A*01阳性后代的产量。 预计第一年的初始队列将产生至少70只婴儿，预计五年内将产生约400只SPF动物的后代。到第六年，该计划应该是自给自足的。 CPRC的计划将为推进生物医学研究做出重大贡献，特别是通过为NIH赞助的研究计划提供高质量和健康的SPF恒河猴来促进艾滋病研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (Provided by applicant): The objective of this proposal is to enhance the existing SPF rhesus macaque supply and breeding colony program at the SSFS of the CPRC. This program will use genetically characterized, major histocompatability complex (MHC)-typed, Indian-origin monkeys from established social groups obtained from the CPRC's free-ranging colony on the island of CS. The CPRC will donate the founding stock for the colony, which consists of 102 adult and 21 juvenile females and minimum of 20 sires. Previous surveys have shown that the Cayo Santiago macaques are free of several important viruses including retroviruses, and that the majority of immature animals are negative for B virus. About 20-25% of these monkeys are also Mamu-A*01 positive. Currently, there is a shortage of SPF rhesus monkeys for biomedical research and the demand for these animals is expected to increase dramatically in the future. The establishment and maintenance of this expanded program at SSFS will help meet the increased demand for both SPF (B virus, SRV-D, SIV, and STLV-l-free) and Mamu-A*01 positive rhesus monkeys. MHC-typing and selective breeding will be used to increase the production of SPF Mamu-A*01 positive offspring. The founding cohort is expected to produce a minimum of 70 infants during the first year and an estimated total offspring of about 400 SPF animals in five years. By Year 6 the program should be self-supporting. The CPRC's program will make a significant contribution to advancing biomedical research, and in particular to AIDS research by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research programs.