ENHANCEMENT OF THE CPRC-SPF RHESUS MONKEY PROGRAM: AIDS IMMUNOLOGY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 描述(由申请人提供)：这项建议的目的是加强中国红十字会SSFS现有的SPF恒河猴供应和繁殖群体计划。该项目将使用从中国红十字会在CS岛上自由放牧的殖民地获得的具有基因特征的主要组织相容性复合体(MHC)类型的印度裔猴子，这些猴子来自现有的社会团体。中国人民政治协商会议将为该殖民地捐赠创始股票，该殖民地由102只成年雌性和21只未成年雌性组成，至少20只雌性。此前的调查表明，圣地亚哥卡约猕猴没有携带包括逆转录病毒在内的几种重要病毒，而且大多数未成熟动物的B病毒呈阴性。这些猴子中约有20%-25%也是MAMU-A*01阳性。目前，用于生物医学研究的SPF恒河猴短缺，预计未来对这些动物的需求将大幅增加。SSFS扩大项目的建立和维持将有助于满足SPF(B病毒、SRV-D、SIV和不含STLV-L)和MAMU-A*01阳性恒河猴日益增长的需求。将采用MHC分型和选择性育种来提高SPF Mamu-A*01阳性后代的产量。创始群体预计在第一年至少生下70只婴儿，五年内估计总后代约为400只SPF动物。到第六年，该计划应该是自给自足的。CPRC的项目将通过向NIH赞助的研究项目提供高质量和健康的SPF恒河猴，为推进生物医学研究，特别是艾滋病研究做出重大贡献。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (Provided by applicant): The objective of this proposal is to enhance the existing SPF rhesus macaque supply and breeding colony program at the SSFS of the CPRC. This program will use genetically characterized, major histocompatability complex (MHC)-typed, Indian-origin monkeys from established social groups obtained from the CPRC's free-ranging colony on the island of CS. The CPRC will donate the founding stock for the colony, which consists of 102 adult and 21 juvenile females and minimum of 20 sires. Previous surveys have shown that the Cayo Santiago macaques are free of several important viruses including retroviruses, and that the majority of immature animals are negative for B virus. About 20-25% of these monkeys are also Mamu-A*01 positive. Currently, there is a shortage of SPF rhesus monkeys for biomedical research and the demand for these animals is expected to increase dramatically in the future. The establishment and maintenance of this expanded program at SSFS will help meet the increased demand for both SPF (B virus, SRV-D, SIV, and STLV-l-free) and Mamu-A*01 positive rhesus monkeys. MHC-typing and selective breeding will be used to increase the production of SPF Mamu-A*01 positive offspring. The founding cohort is expected to produce a minimum of 70 infants during the first year and an estimated total offspring of about 400 SPF animals in five years. By Year 6 the program should be self-supporting. The CPRC's program will make a significant contribution to advancing biomedical research, and in particular to AIDS research by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research programs.