BioCurrents Research Center

生物电流研究中心是NCRR的一个资源，专门研究离子的传输， 穿过细胞质膜的小分子。一个特殊的专长在于应用 电化学微传感器来描述扩展边界层内的扩散，并因此定义了 细胞状态的化学特征微传感器是非侵入性的，电位和电流， 微米比例，并且通常以调制格式操作。分子和离子的测量可以 从异质群体中鉴定的单细胞制成，并且可以从特定区域的细胞中取样。 细胞可以遵循缓慢和非产电转运事件。修饰微电极及其 操作允许基于离子扩散非侵入性地检测毫秒通道事件 从细胞膜上的来源。下一个周期的拟议核心目标是： 单细胞通道检测和分析以及反馈位置控制;开发多分析物 传感器，以促进转运蛋白的化学计量分析和同心电极设计，以调节 围绕传感器的流体微体积;开发低光成像技术，靶向功能化精细 颗粒和质粒报告基因，以监测细胞外直接的化学特征， 细胞间隙;用于卵巢癌临床应用简化和自动化电化学平台 癌症和生殖医学。核心目标的选择是为了提供新的方法， 推动生物医学项目，这些项目涵盖转运蛋白和细胞功能方面的基本生物医学兴趣， 细胞和组织病理学中清晰的临床问题。为了实现NIH资源的目标，我们 还提供广泛的协作和服务组件。我们的大多数用户群收到 基金来自美国国立卫生研究院，但我们也接待来自其他联邦机构，如国家科学基金会和国防部的利益。 这些活动沿着我们通过培训和支持校外研究活动的推广， 在下一个周期中得到加强。

The Biocurrents Research Center, an NCRR resource, specializes in studying the transport of ions and nnolecules across the plasma membrane of cells. A particular expertise lies in the application of electrochemical microsensors to describing diffusion within the extended boundary layer and thus defining a chemical signature of the cell state. Microsensors are non-invasive, potentiometric and amperometric, of micron proportions, and normally operate in a modulation format. Measurement of molecules and ions can be made from identified single cells in heterogeneous populations, and can sample from specific regions of cells. Slow and non-electrogenic transport events can be followed. Modifying the microelectrode and its operation allows the detection of millisecond channel events, non-invasively, on the basis of ion diffusion from the source on the cell membrane. Proposed core objectives for the next cycle are to: further develop channel detection and analysis from single cells alongside feedback position control; develop multianalyte sensors to facilitate stochiometric analysis of transporters and concentric electrode designs to regulate the fluid micro-volumes surrounding sensors; develop low light imaging techniques, targeting functionalized fine particles and plasmid reporters, to monitor the chemical signatures within the immediate extracellular and intercellular space; simplify and automate electrochemical platforms for clinical applications in ovarian cancer and reproductive medicine. The Core Objectives are chosen to provide new methodologies to the Driving Biomedical Projects which span basic biomedical interests in transporters and cellular function, to clear clinical questions in cellular and tissue pathology. In keeping with the goals of an NIH resource, we also offer an extensive collaborative and service component. The large majority of our user group receives funds from the NIH but we also host interests from other federal agencies, such as the NSF and DoD. These activities along with our outreach through training and support of off-campus research initiatives will be enhanced through the next cycle.