BioCurrents Research Center

生物电流研究中心是NCRR的一个资源，专门研究离子和 穿过细胞质膜的小分子。一项特殊的专业知识在于应用 用于描述扩展边界层内的扩散的电化学微传感器，从而定义 细胞状态的化学特征。微型传感器是非侵入式的、电位型和电流型。 微米比例，并且通常以调制格式运行。分子和离子的测量可以 是由不同种群中已鉴定的单个细胞制成的，可以从特定的区域采集样本 细胞。可以跟随缓慢且不产生电的传输事件。微电极的改性及其应用 操作允许在离子扩散的基础上非侵入性地检测毫秒通道事件 来自细胞膜上的源头。下一个周期的拟议核心目标是：进一步发展 从单细胞进行通道检测和分析，同时进行反馈位置控制；开发多分析物 便于转运体化学计量分析的传感器和调节 传感器周围的流体微体积；开发微光成像技术，靶向功能化精细 颗粒和质粒记者，监测直接细胞外和细胞内的化学特征 细胞间隙；简化和自动化卵巢临床应用的电化学平台 癌症和生殖医学。选择核心目标是为了向 推动生物医学项目，这些项目涉及转运蛋白和细胞功能方面的基本生物医学兴趣，以 明确细胞和组织病理学的临床问题。为了与NIH资源的目标保持一致，我们 还提供广泛的协作和服务组件。我们的绝大多数用户群收到 资金来自NIH，但我们也有来自其他联邦机构的利益，如NSF和国防部。 这些活动以及我们通过培训和支持校外研究倡议进行的外联活动将 在下一个周期中得到加强。

The Biocurrents Research Center, an NCRR resource, specializes in studying the transport of ions and nnolecules across the plasma membrane of cells. A particular expertise lies in the application of electrochemical microsensors to describing diffusion within the extended boundary layer and thus defining a chemical signature of the cell state. Microsensors are non-invasive, potentiometric and amperometric, of micron proportions, and normally operate in a modulation format. Measurement of molecules and ions can be made from identified single cells in heterogeneous populations, and can sample from specific regions of cells. Slow and non-electrogenic transport events can be followed. Modifying the microelectrode and its operation allows the detection of millisecond channel events, non-invasively, on the basis of ion diffusion from the source on the cell membrane. Proposed core objectives for the next cycle are to: further develop channel detection and analysis from single cells alongside feedback position control; develop multianalyte sensors to facilitate stochiometric analysis of transporters and concentric electrode designs to regulate the fluid micro-volumes surrounding sensors; develop low light imaging techniques, targeting functionalized fine particles and plasmid reporters, to monitor the chemical signatures within the immediate extracellular and intercellular space; simplify and automate electrochemical platforms for clinical applications in ovarian cancer and reproductive medicine. The Core Objectives are chosen to provide new methodologies to the Driving Biomedical Projects which span basic biomedical interests in transporters and cellular function, to clear clinical questions in cellular and tissue pathology. In keeping with the goals of an NIH resource, we also offer an extensive collaborative and service component. The large majority of our user group receives funds from the NIH but we also host interests from other federal agencies, such as the NSF and DoD. These activities along with our outreach through training and support of off-campus research initiatives will be enhanced through the next cycle.