BioCurrents Research Center

生物电流研究中心

• 批准号: 7991935
• 负责人: PETER JS SMITH
• 金额: $ 98.06万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7991935
• 关键词: Address; Animals; Automobile Driving; Back; Caliber; Cell membrane; Cell physiology; Cells; Chemicals; Clinical; Complex; Detection; Development; Diffusion; Disease; Electrodes; Engineering; Environment; Event; Extracellular Space; Feedback; Funding; Goals; Heart; Imaging Techniques; Ion Transport; Ions; Light; Liquid substance; Malignant neoplasm of ovary; Measurement; Measures; Membrane; Methodology; Methods; Microelectrodes; Monitor; National Center for Research Resources; Normal Cell; Pathology; Performance; Physiology; Plants; Plasmids; Population Heterogeneity; Positioning Attribute; Publications; Regulation; Reporter; Reproductive Medicine; Research; Resources; Sampling; Science; Services; Signal Transduction; Source; Techniques; Tissues; Training Support; United States National Institutes of Health; base; clinical application; design; extracellular; feeding; innovation; interest; millisecond; nano; operation; outreach; particle; programs; release of sequestered calcium ion into cytoplasm; sensor; single cell analysis

The Biocurrents Research Center, an NCRR resource, specializes in studying the transport of ions and nnolecules across the plasma membrane of cells. A particular expertise lies in the application of electrochemical microsensors to describing diffusion within the extended boundary layer and thus defining a chemical signature of the cell state. Microsensors are non-invasive, potentiometric and amperometric, of micron proportions, and normally operate in a modulation format. Measurement of molecules and ions can be made from identified single cells in heterogeneous populations, and can sample from specific regions of cells. Slow and non-electrogenic transport events can be followed. Modifying the microelectrode and its operation allows the detection of millisecond channel events, non-invasively, on the basis of ion diffusion from the source on the cell membrane. Proposed core objectives for the next cycle are to: further develop channel detection and analysis from single cells alongside feedback position control; develop multianalyte sensors to facilitate stochiometric analysis of transporters and concentric electrode designs to regulate the fluid micro-volumes surrounding sensors; develop low light imaging techniques, targeting functionalized fine particles and plasmid reporters, to monitor the chemical signatures within the immediate extracellular and intercellular space; simplify and automate electrochemical platforms for clinical applications in ovarian cancer and reproductive medicine. The Core Objectives are chosen to provide new methodologies to the Driving Biomedical Projects which span basic biomedical interests in transporters and cellular function, to clear clinical questions in cellular and tissue pathology. In keeping with the goals of an NIH resource, we also offer an extensive collaborative and service component. The large majority of our user group receives funds from the NIH but we also host interests from other federal agencies, such as the NSF and DoD. These activities along with our outreach through training and support of off-campus research initiatives will be enhanced through the next cycle.

生物电流研究中心是NCRR的一个资源，专门研究离子和离子的传输

项目成果

Bioelectric mechanisms in regeneration: Unique aspects and future perspectives.

• DOI: 10.1016/j.semcdb.2009.04.013
• 发表时间: 2009-07
• 期刊: Seminars in cell & developmental biology
• 影响因子: 7.3
• 作者: Levin M

The vibrating Ca2+ electrode: a new technique for detecting plasma membrane regions of Ca2+ influx and efflux.

• DOI: 10.1016/s0091-679x(08)61112-7
• 发表时间: 1994
• 期刊: Methods in cell biology
• 作者: P. Smith;R. Sanger;L. Jaffe

Net calcium and acid release at fertilization in eggs of sea urchins and ascidians.

海胆和海鞘卵受精时的净钙和酸释放。

• DOI: 10.1016/0143-4160(93)90020-7
• 发表时间: 1993
• 期刊: Cell calcium
• 影响因子: 4
• 作者: Kühtreiber,WM;Gillot,I;Sardet,C;Jaffe,LF
• 通讯作者: Jaffe,LF

Sustaining olfaction at low salinities: evidence for a paracellular route of ion movement from the hemolymph to the sensillar lymph in the olfactory sensilla of the blue crab Callinectes sapidus.

在低盐度下维持嗅觉：蓝蟹 Callinectes sapidus 嗅觉感受器中从血淋巴到感器淋巴的离子运动的细胞旁途径的证据。

• DOI: 10.1007/s004410000246
• 发表时间: 2000
• 期刊: Cell and tissue research
• 影响因子: 3.6
• 作者: Gleeson,RA;McDowell,LM;Aldrich,HC;Hammar,K;Smith,PJ
• 通讯作者: Smith,PJ

Tagging and tracking individual networks within a complex mitochondrial web with photoactivatable GFP.

使用可光激活的 GFP 标记和跟踪复杂线粒体网络中的各个网络。

• DOI: 10.1152/ajpcell.00348.2005
• 发表时间: 2006
• 期刊: American journal of physiology. Cell physiology
• 作者: Twig,Gilad;Graf,SolomonA;Wikstrom,JakobD;Mohamed,Hibo;Haigh,SarahE;Elorza,Alvaro;Deutsch,Motti;Zurgil,Naomi;Reynolds,Nicole;Shirihai,OrianS
• 通讯作者: Shirihai,OrianS