MT VET COBRE II CORE B: CELLULAR ANALYSIS CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 细胞分析核心(核心B)由Mark Jutila博士指导，提供技术专长和最先进的仪器，用于使用流式细胞仪、标准和共聚焦荧光显微镜以及免疫组织化学分析生物样本。Cobre II中的所有单独项目都使用核心B提供和开发的资源、专业知识和技术来回答与各自模型系统相关的特定科学问题。 科布雷II 本报告所述期间是在从科布雷一号向科布雷二号过渡期间重新开发和重新设计的核心B的第二年。在科布雷一号期间，细胞分析核心(当时称为核心C)，特别是流式细胞仪设施，是中心内使用最多的核心设施资源。每年平均有50个用户，密歇根州立大学由科布雷资助的每个项目负责人在他们的研究项目中都使用了这个核心。根据Cobre i EAC的建议，我们专注于扩大和增强流式细胞仪设施的能力，购置了四台新的流式细胞仪，其中三台采用了最先进的数字技术。在Cobre II中，我们扩展了细胞分析核心的能力，增加了一个组织学部分，并将其重新命名为“核心B”，如本报告所述期间所示。与这个核心的早期时代一样，细胞分析核心继续为Cobre II计划的成员以及其他与中心相关的免疫学和传染病(在过去的报告中称为“兽医分子生物学”)和密歇根州立大学的其他部门提供服务、指导和咨询。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Cellular Analysis Core (Core B) is directed by Dr. Mark Jutila and provides technical expertise and state-of-the-art instrumentation for analysis of biological samples using flow cytometry, standard and confocal fluorescence microscopy, and immunohistochemistry. All of the individual projects in COBRE II use the resources, expertise, and techniques provided and developed by Core B to answer specific scientific questions related to their individual model systems. COBRE II This reporting period represented the second year of the redeveloped and redesigned Core B, which occurred during the transition from COBRE I to COBRE II. During COBRE I, the Cellular Analysis Core (then called Core C) and in particular, the Flow Cytometry Facility, represented the most used Core facility resource within the Center. The number of yearly users averaged 50/yr, and each of the COBRE-funded Project Leaders at MSU used this Core in their research projects. Following recommendations by the COBRE I EAC, we focused on expanding and enhancing the capabilities of the flow cytometry facility, acquiring four new flow cytometers, including three with state-of-the-art digital-based technologies. In COBRE II, we expanded the capabilities of the Cellular Analysis Core by adding a histology component and renamed it "Core B" as represented in the current reporting period. As with the earlier era of this Core, the Cellular Analysis Core continued to provide service, instruction, and consultation to members of the COBRE II program, as well as to other Center-associated investigators in Immunology and Infectious Diseases (called "Veterinary Molecular Biology" in past reports) and other MSU departments.