MT VET COBRE II CORE B: CELLULAR ANALYSIS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞分析核心(核心B)由Mark Jutila博士指导，提供技术专长和最先进的仪器，用于使用流式细胞仪、标准和共聚焦荧光显微镜以及免疫组织化学分析生物样本。Cobre II中的所有单独项目都使用核心B提供和开发的资源、专业知识和技术来回答与各自模型系统相关的特定科学问题。 科布雷一号到科布雷二号的过渡 核心B的发展发生在从Cobre I向Cobre II的过渡期间。在Cobre I期间，细胞分析核心(当时称为核心C)，特别是流式细胞仪设备，代表了中心内使用最多的核心设施资源。每年平均有50名用户，密歇根州立大学由科布雷资助的每个项目负责人在他们的研究项目中都使用了这个核心。根据Cobre i EAC的建议，我们在过去三年专注于扩大和加强流式细胞仪设施的能力，购置了四台新的流式细胞仪，其中三台采用了最先进的数码技术。在Cobre II中，我们扩展了细胞分析核心的能力，增加了一个组织学部分，并将其重新命名为“核心B”，如本报告所述期间所示。这是我们增强和维持分析体内细胞反应能力的长期计划的第一步。这将补充我们目前主要专注于体外和体外细胞分析的能力。与Cobre I计划一样，细胞分析核心为Cobre II计划成员以及兽医分子生物学和其他密歇根州立大学部门的其他与中心相关的研究人员提供服务、指导和咨询。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cellular Analysis Core (Core B) is directed by Dr. Mark Jutila and provides technical expertise and state-of-the-art instrumentation for analysis of biological samples using flow cytometry, standard and confocal fluorescence microscopy, and immunohistochemistry. All of individual projects in COBRE II use the resources, expertise, and techniques provided and developed by Core B to answer specific scientific questions related to their individual model systems. COBRE I to COBRE II Transition The development of Core B occurred during the transition from COBRE I to COBRE II. During COBRE I, the Cellular Analysis Core (then called Core C) and in particular, the Flow Cytometry Facility, represented the most used Core facility resource within the Center. The number of yearly users averaged 50/yr, and each of the COBRE funded Project Leaders at MSU used this Core in their research projects. Following recommendations by the COBRE I EAC, we focused in the past three years on expanding and enhancing capabilities of the flow cytometry facility, acquiring four new flow cytometers, including three with state-of-the-art digital-based technologies. In COBRE II, we expanded the capabilities of the Cellular Analysis Core by adding a histology component and renamed it "Core B" as represented in the current reporting period. This represented the first step in our long-range plan of enhancing and sustaining our capability to analyze cellular responses in vivo. This will complement our current capabilities, which are primarily focused on in vitro and ex vivo cellular analyses. As under COBRE I, the Cellular Analysis Core provided service, instruction, and consultation to members of the COBRE II program, as well as to other Center-associated investigators in Veterinary Molecular Biology and other MSU departments.