MT VET COBRE II CORE B: CELLULAR ANALYSIS CORE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 细胞分析核心（核心B）由Mark Jutila博士指导，为使用流式细胞术、标准和共聚焦荧光显微镜和免疫组织化学分析生物样品提供技术专业知识和最先进的仪器。COBRE II中的所有单个项目都使用Core B提供和开发的资源、专业知识和技术来回答与其单个模型系统相关的特定科学问题。 COBRE I到COBRE II的过渡 核心B的开发发生在从COBRE I到COBRE II的过渡期间。在COBRE I期间，细胞分析核心（当时称为核心C），特别是流式细胞仪，代表了中心内使用最多的核心设施资源。每年的用户数量平均为50/年，每个COBRE资助的项目领导人在密歇根州立大学使用这个核心在他们的研究项目。根据COBRE I EAC的建议，我们在过去三年中专注于扩大和增强流式细胞仪设施的能力，购买了四台新的流式细胞仪，其中三台采用了最先进的数字技术。在COBRE II中，我们通过添加组织学组件扩展了细胞分析核心的功能，并将其重命名为“核心B”，如本报告期间所示。这代表了我们增强和维持体内细胞反应分析能力的长期计划的第一步。这将补充我们目前的能力，主要集中在体外和离体细胞分析。与COBRE I一样，细胞分析核心为COBRE II计划的成员以及兽医分子生物学和其他MSU部门的其他中心相关研究人员提供服务，指导和咨询。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cellular Analysis Core (Core B) is directed by Dr. Mark Jutila and provides technical expertise and state-of-the-art instrumentation for analysis of biological samples using flow cytometry, standard and confocal fluorescence microscopy, and immunohistochemistry. All of individual projects in COBRE II use the resources, expertise, and techniques provided and developed by Core B to answer specific scientific questions related to their individual model systems. COBRE I to COBRE II Transition The development of Core B occurred during the transition from COBRE I to COBRE II. During COBRE I, the Cellular Analysis Core (then called Core C) and in particular, the Flow Cytometry Facility, represented the most used Core facility resource within the Center. The number of yearly users averaged 50/yr, and each of the COBRE funded Project Leaders at MSU used this Core in their research projects. Following recommendations by the COBRE I EAC, we focused in the past three years on expanding and enhancing capabilities of the flow cytometry facility, acquiring four new flow cytometers, including three with state-of-the-art digital-based technologies. In COBRE II, we expanded the capabilities of the Cellular Analysis Core by adding a histology component and renamed it "Core B" as represented in the current reporting period. This represented the first step in our long-range plan of enhancing and sustaining our capability to analyze cellular responses in vivo. This will complement our current capabilities, which are primarily focused on in vitro and ex vivo cellular analyses. As under COBRE I, the Cellular Analysis Core provided service, instruction, and consultation to members of the COBRE II program, as well as to other Center-associated investigators in Veterinary Molecular Biology and other MSU departments.