UNDERSTANDING AND CONTROLLING TISSUE-SPECIFIC VASCULAR PATTERNING

了解和控制组织特异性血管模式

• 批准号: 8360198
• 负责人: SUSAN M LESSNER
• 金额: $ 20.95万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360198
• 关键词: Architecture; Binding; Biocompatible Materials; Blood Vessels; Blood capillaries; Cells; Developmental Process; Diabetic Retinopathy; Embryo; Embryonic Development; Endothelial Cells; Engineering; Ensure; Extracellular Matrix; Fibroblast Growth Factor; Funding; Goals; Grant; Growth; Growth Factor; Heparin; Hydrogels; In Vitro; Lead; Malignant Neoplasms; Methods; Mus; National Center for Research Resources; Pattern; Principal Investigator; Process; Research; Research Infrastructure; Resources; Role; Source; Structure; Tissue Engineering; Tissues; United States National Institutes of Health; Vascular Endothelial Growth Factors; Vascular blood supply; Vascularization; capillary; capillary bed; cost; cytokine; improved; in vivo; research study; tissue regeneration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During embryonic development, capillary networks develop and remodel in an orderly pattern to ensure adequate blood supply to growing tissues. The architecture of developing capillary beds is tissue-specific, reflecting differences in the structure and function of the surrounding cells. This orderly process is disrupted in pathological conditions such as cancer and diabetic retinopathy. In tissue engineering, controlling vascularization of engineered constructs in a way which recapitulates developmental processes remains a desirable but elusive goal. In vivo, gradients of growth factors bound to the extracellular matrix have been implicated in directing capillary patterning in the developing mouse embryo. We are developing heparin-modified hydrogels which can present growth factors such as VEGF or FGF to endothelial cells in a well-controlled fashion, to investigate the role of matrix-bound and free cytokine gradients in controlling vascular patterning. The project involves materials synthesis and characterization as well as in vitro experiments with endothelial cells. These studies may lead to a better understanding of tissue-specific vascular patterning during embryonic development as well as to improved methods to promote functional blood vessel growth in tissue-engineered constructs.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在胚胎发育过程中，毛细血管网络以有序的模式发育和重塑，以确保生长组织有足够的血液供应。 发育中的毛细血管床的结构具有组织特异性，反映了周围细胞结构和功能的差异。 这种有序的过程在癌症和糖尿病视网膜病变等病理条件下被破坏。 在组织工程中，以再现发育过程的方式控制工程构建物的血管化仍然是一个理想但难以实现的目标。 在体内，生长因子结合到细胞外基质的梯度已经涉及在发育中的小鼠胚胎中指导毛细血管图案化。 我们正在开发肝素改性的水凝胶，它可以目前的生长因子，如VEGF或FGF的内皮细胞在一个良好的控制方式，调查的作用，控制血管图案的基质结合和自由的细胞因子梯度。 该项目涉及材料合成和表征以及内皮细胞的体外实验。 这些研究可能会导致更好地了解胚胎发育过程中的组织特异性血管模式，以及改进的方法，以促进组织工程结构中的功能性血管生长。