UNDERSTANDING AND CONTROLLING TISSUE-SPECIFIC VASCULAR PATTERNING

了解和控制组织特异性血管模式

• 批准号: 8168473
• 负责人: SUSAN M LESSNER
• 金额: $ 25.62万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168473
• 关键词: Architecture; Binding; Blood Vessels; Blood capillaries; Cells; Computer Retrieval of Information on Scientific Projects Database; Developmental Process; Diabetic Retinopathy; Embryo; Embryonic Development; Endothelial Cells; Engineering; Ensure; Extracellular Matrix; Fibroblast Growth Factor; Funding; Goals; Grant; Growth; Growth Factor; Heparin; Hydrogels; In Vitro; Institution; Lead; Malignant Neoplasms; Methods; Mus; Pattern; Process; Research; Research Personnel; Resources; Role; Source; Structure; Tissue Engineering; Tissues; United States National Institutes of Health; Vascular Endothelial Growth Factors; Vascular blood supply; Vascularization; capillary; capillary bed; cytokine; improved; in vivo; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During embryonic development, capillary networks develop and remodel in an orderly pattern to ensure adequate blood supply to growing tissues. The architecture of developing capillary beds is tissue-specific, reflecting differences in the structure and function of the surrounding cells. This orderly process is disrupted in pathological conditions such as cancer and diabetic retinopathy. In tissue engineering, controlling vascularization of engineered constructs in a way which recapitulates developmental processes remains a desirable but elusive goal. In vivo, gradients of growth factors bound to the extracellular matrix have been implicated in directing capillary patterning in the developing mouse embryo. We are developing heparin-modified hydrogels which can present growth factors such as VEGF or FGF to endothelial cells in a well-controlled fashion, to investigate the role of matrix-bound and free cytokine gradients in controlling vascular patterning. The project involves materials synthesis and characterization as well as in vitro experiments with endothelial cells. These studies may lead to a better understanding of tissue-specific vascular patterning during embryonic development as well as to improved methods to promote functional blood vessel growth in tissue-engineered constructs.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在胚胎发育过程中，毛细血管网络以有序的方式发育和重塑，以确保生长组织有足够的血液供应。 发育中的毛细血管床的结构是组织特异性的，反映了周围细胞的结构和功能的差异。 这种有序的过程在癌症和糖尿病视网膜病变等病理情况下被破坏。 在组织工程中，以概括发育过程的方式控制工程构建体的血管化仍然是一个理想但难以实现的目标。 在体内，与细胞外基质结合的生长因子的梯度与指导发育中的小鼠胚胎中的毛细血管图案有关。 我们正在开发肝素修饰的水凝胶，它可以以良好控制的方式将 VEGF 或 FGF 等生长因子呈现给内皮细胞，以研究基质结合和游离细胞因子梯度在控制血管模式中的作用。 该项目涉及材料合成和表征以及内皮细胞体外实验。 这些研究可能有助于更好地了解胚胎发育过程中的组织特异性血管模式，并改进促进组织工程结构中功能性血管生长的方法。