Viral Sequencing Core

描述CORE J与现场调查人员以及CHAVI内部其他人员之间的互动，以解释 目标以及这些不同小组可能协同工作的方式。 CoreJ调查人员产生的结果将在每周CHAVI上定期讨论 电话会议。因此，临床现场和其他CHAVI调查人员敏锐地了解相关数据， 问题和问题，并就向前推进进行协商。中的此类交互示例 过去一年包括：(I)临床研究人员从慢性感染病人中挑选样本，作为 用于急性感染分析的对照，(Ii)选择第一组功能性环境克隆用于进一步 生物学特征，(3)选择急性感染样本的子集以进行更详细的纵向分析 准种分析，以及(Iv)用于CTL全长基因组分析的急性感染样本的选择 学习。 不同的测序点如何监测绩效指标(UAB和南非)？是谁 在这两个站点之间做什么？ 在过去的预算期间，南非网站的重点是急性胰腺炎的基因特征。 和慢性C亚型感染，而UAB，Duke和UNC站点已经完成了第一个全面的 急性乙型亚型感染分析。在未来的一年里，南非和北卡罗来纳大学将负责 为完成南非和马拉维急性C亚型感染的基因特征， 而UAB和Duke将专注于对急性感染样本的纵向分析，全长基因组 序列分析和功能克隆的产生。优先顺序和工作量在 按季度计算。CHAVI特别工作组审查了个别地点的进展情况，预算调整如下 在必要的时候制作。

Describe the interactions between Core J and site investigators and others within CHAVI to explain the goals and the ways in which these various groups might work synergistically. Results generated by Core J investigators are discussed on a regular basis at the weekly CHAVI conference calls. Thus, clinical site and other CHAVI investigators are acutely aware of pertinent data, questions and issues, and are consulted with respect to moving forward. Examples of such interactions in the past year included: (i) selection of samples from chronically infected patients by clinical investigators as controls for the acute infection analysis, (ii) selection of a first set of functional env clones for further biological characterization, (iii) selection of a subset of acute infection samples for more detailed longitudinal quasispecies analyses, and (iv) selection of acute infection samples for full-length genome analysis for CTL studies. How are the different sequencing sites monitoring performance metrics (UAB and South Africa)? Who is doing what between these two sites? In the past budget period, the South African site has focused on the genetic characterization of acute and chronic subtype C infections, while UAB, Duke and UNC sites have completed the first comprehensive analysis of acute subtype B infections. In the coming year, South Africa and UNC sites will be responsible for the completion of the genetic characterization of acute subtype C infections in South Africa and Malawi, while UAB and Duke will focus on longitudinal analyses of acute infection samples, full length genome sequence analysis, and generation of functional clones. Priorities and workload are discussed on a quarterly basis. Progress at individual sites is reviewed by the CHAVI SLG and budgetary adjustments are made when necessary.