CIRCUIT ANALYSIS OF PFC-MTL INTERACTIONS IN RATS

大鼠 PFC-MTL 相互作用的电路分析

• 批准号: 8303574
• 负责人: Howard B. Eichenbaum
• 金额: $ 24.14万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-06 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8303574
• 关键词: Area; Cognition; Complement; Computer Simulation; Data; Development; Electrodes; Elements; Generations; Hippocampus (Brain); Human; Instruction; Ipsilateral; Learning; Light; Link; Literature; Medial; Memory; Mental disorders; Methods; Modeling; Monitor; Monkeys; Muscimol; Nature; Neurons; Odors; Optics; Pattern; Positioning Attribute; Process; Property; Rattus; Resolution; Retrieval; Rewards; Rodent; Rodent Model; Techniques; Testing; Time; base; entorhinal cortex; information processing; millisecond; neural information processing; relating to nervous system; research study

PROJECT SUMMARY (See Instructions): This project will employ a combination of recording and reversible inactivation techniques to examine how PFC and MTL areas interact in rats performing a context-guided association task that is formally equivalent to the test used in humans and monkeys in other projects. In this task, on each trial a rat will be move into one of two environmental contexts that conditions associations between odor stimulis and rewards. Using multichannel tetrodes, we will record simultaneously in an MTL area and a PFC area, and test specific hypotheses outlined about the development, nature, timing, and coordination of firing patterns in these areas. These studies will test the generality of our overall hypotheses about information processing in PFC and MTL areas in rats as compared to those in humans and monkeys examined in Projects 1-3. We will also go beyond identifying functional properties of neuronal activity in other experiments by simultaneously recording in one area while reversibly inactivating another area for brief periods during and after learning. Reversible inactivation will be accomplished using florescent muscimoi and by state-of-the-art optical silencing methods that allow millisecond resolution of anatomically identified areas. These experiments will provide tests of whether firing patterns normally observed in one area depend on the other. For example, we will test the hypothesis that, during learning, the development of context general representations in PFC depend on specific MTL areas; whether, within trials following learning, the generation of specific context representations in postrhinal/medial entorhinal cortex depends on PFC; and whether the generation of specific representations of the second element on a conditional association in PFC depends on perirhinal cortex. These and other specific tests will provide evidence on causal relations among areas within PFCMTL circuitry, providing key evidence for computational modeling in Project 6. This project will closely parallel Project 5, which examines the nature of PFC-MTL interactions in rats performing a similar context guided spatial maze task, thus allowing direct comparisons that link the PFC-MTL processing of non-spatial memorties with the large literature on hippocampal spatial memory.

项目摘要（请参阅说明）： 该项目将采用记录和可逆的灭活技术的组合来研究PFC和MTL区域如何在大鼠中进行上下文引导的关联任务的相互作用，该任务与其他项目中的人类和猴子中使用的测试正式等同。在此任务中，在每次试验中，一只大鼠将被转移到条件的两个环境环境之一中，该环境刺激和奖励之间存在关联。使用多通道四极管，我们将同时在MTL区域和PFC区域记录，并测试有关这些区域中射击模式的开发，性质，时机和协调的特定假设。 与项目1-3中检查的人类和人类和猴子相比，这些研究将检验我们关于PFC和MTL地区信息处理的总体假设的普遍性。我们还将超越其他实验中通过同时识别神经元活动的功能特性 在学习期间和之后，在一个区域记录一个区域，同时在短时间内不可逆地灭活另一个区域。 可逆性的失活将使用荧光的肌肉菌和最先进的光沉默方法来实现，从而允许毫秒解析解剖学上的区域。这些实验将提供有关在一个区域中通常观察到的点火模式是否取决于另一个区域的测试。例如，我们将检验以下假设：在学习过程中，PFC中上下文一般表示的发展取决于特定的MTL领域。在学习后的试验中，是否在后/内侧内部皮层中生成特定上下文表示的生成取决于PFC。第二个元素在PFC中的条件关联上的特定表示的产生是否取决于周围的皮质。这些和其他特定的测试将提供有关PFCMTL电路领域之间因果关系的证据，为项目6中的计算建模提供了关键证据。该项目将紧密平行的项目5，该项目研究了PFC-MTL相互作用的性质空间内存。