INBRE: URI: CENTRALIZED RES CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 RI-INBRE集中研究核心设施向网络参与者和罗得岛生物医学研究界提供研究和培训支助已进入第七个年头。 在本赠款期内，超过57个研究小组使用了该设施，平均每季度累计使用次数为1 200次。 该设施的用户包括32名教师，31名博士后研究员，93名研究生和20名本科生。 该基金工作人员向所有新用户提供培训和咨询，以便在其研究项目中更好地利用各种技术。 此外，工作人员继续参与教授药学院提供的本科生和研究生实验室课程的各种技术。 这些课程旨在为使用该设施设备的学生提供理论和实践经验。 在这一年中，与仪器制造商合作举办了几次教学研讨会和培训讲习班。这些专题包括：“使用SELDI-TOF技术发现和表征新的生物标志物”;“Xcelligence系统是无标记、基于细胞凋亡和GPCR侵入迁移的真实的时间细胞分析的新前沿”;“小分子环境中的QSTAR-MS/MS”;“带电气溶胶检测器（CAD）是一种用于常规测定任何非挥发性化学物质的新型质量敏感通用检测器;和“在EPA ToxCast计划中使用CDAS安全毒理学平台分析化学毒性”。 根据RI-IBRE参与者的建议，今年购买的新设备包括：快速基因810核酸分离系统; Polarstar Optima酶标仪和Cellometer Vision自动细胞计数器。SEDI-TOF系统已经过时，因为BioRad已经停止了未来的生产和升级。 该工厂将寻求用具有SELDI功能的新TOF-TOF机器取代该系统。 总共印刷了170张海报，非RI-INBRE研究人员支付适度的费用来支付消耗品的费用。继续免费向研究人员提供设备和咨询。总体而言，核心设施仪器及其咨询服务的使用继续增加。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The RI-INBRE Centralized Research Core Facility is in its seventh year of providing research and training support to the Network participants and the Rhode Island biomedical research community. During the current grant period more than 57 research groups have used the Facility, with an average quarterly cumulative frequency of 1,200 usages. The Facility users consisted of 32 faculty, 31 postdoctoral fellows, 93 graduate students, and 20 undergraduate students. The Facility staff provided training and consultation to all new users and in order to better utilize various technologies in their research projects. In addition, the staff continued to participate in teaching various techniques in the undergraduate and graduate laboratory courses offered by the College of Pharmacy. These courses are designed to provide both theoretical and hands-on experience to students using the Facility's equipment. Throughout the year, several instructional seminars and training workshops were organized in collaboration with the instrument manufacturers. The topics included: "Discovery and characterization of novel biomarkers using SELDI-TOF technology"; "Xcelligence System a new frontier in label free, real time cell based assays for apoptosis, and GPCR invasion migration"; " QSTAR-MS/MS in a small molecule environment"; " Charged Aerosol Detector (CAD) a novel mass sensitive universal detector for the routine determination of any non-volatile chemical species; and "Profiling chemical toxicity using CDAS safety toxicology platform in the EPA ToxCast program". Based on the recommendations received from the RI-IBRE participants, new equipment acquired this year included the following: Quick Gene 810 nucleic acid isolation system; Polarstar Optima microplate reader' and Cellometer Vision automated cell counter. The SEDI-TOF system has become obsolete because BioRad has discontinued future production and upgrade. The Facility will be looking to replace this system with a new TOF-TOF machine with SELDI capability. A total of 170 posters were printed with non-RI-INBRE researchers paying a modest fee to cover the cost of consumables. The equipment and consultation were continued to be made available to the researchers at no cost. Overall, the usage of the Core Facility instrumentation and its consultative services has continued to increase.